PMID- 19720069
OWN - NLM
STAT- MEDLINE
DCOM- 20100108
LR  - 20181201
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 671
IP  - 1-2
DP  - 2009 Dec 1
TI  - Differential regulation of the hydrogen-peroxide-induced inhibition of 
      gap-junction intercellular communication by resveratrol and butylated 
      hydroxyanisole.
PG  - 40-4
LID - 10.1016/j.mrfmmm.2009.08.011 [doi]
AB  - The present study was performed to evaluate the effects of two different phenolic 
      antioxidants, resveratrol (3,5,4'-trihydroxystilbene) and butylated 
      hydroxyanisole (BHA), on the hydrogen peroxide (H2O2)-induced inhibition of 
      gap-junction intercellular communication (GJIC) in WB-F344 rat liver epithelial 
      cells (WB-F344). Resveratrol is a naturally occurring polyphenolic antioxidant; 
      on the other hand, BHA is a synthetic phenolic compound. We found that only 
      resveratrol protects WB-F344 cells from H2O2-induced inhibition of GJIC, and BHA 
      has no effect. The extracellular-signal-regulated protein kinase 1/2 
      (ERK1/2)-connexin 43 (Cx43) signaling pathway is crucial for the regulation of 
      GJIC in rat liver epithelial cells, and resveratrol, but not BHA, blocked the 
      H2O2-induced phosphorylation of Cx43, a critical regulator of GJIC, and ERK1/2 in 
      WB-F344 cells. Resveratrol appears to attenuate the H2O2-mediated ERK1/2-Cx43 
      signaling pathway and consequently reverses H2O2-mediated inhibition of GJIC. 
      DPPH and ABTS radical-scavenging assays revealed that the protective effect of 
      resveratrol on the H2O2-mediated inhibition of GJIC was not mediated through its 
      free radical-scavenging activity.
FAU - Kim, Jong Hun
AU  - Kim JH
AD  - Major in Biomodulation, Department of Agricultural Biotechnology, Research 
      Institute for Agriculture and Life Sciences, Seoul National University, 599 
      Gwangak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea.
FAU - Choi, Seung Hwan
AU  - Choi SH
FAU - Kim, Jiyoung
AU  - Kim J
FAU - Lee, Bo Kyung
AU  - Lee BK
FAU - Lee, Ki Won
AU  - Lee KW
FAU - Lee, Hyong Joo
AU  - Lee HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090829
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antioxidants)
RN  - 0 (Connexin 43)
RN  - 0 (Free Radicals)
RN  - 0 (Stilbenes)
RN  - 25013-16-5 (Butylated Hydroxyanisole)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Butylated Hydroxyanisole/*pharmacology
MH  - Cell Communication/*drug effects
MH  - Cell Line
MH  - Connexin 43/metabolism
MH  - Free Radicals/metabolism
MH  - Gap Junctions/drug effects/*physiology
MH  - Hydrogen Peroxide/*pharmacology
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Inbred F344
MH  - Resveratrol
MH  - Signal Transduction/drug effects
MH  - Stilbenes/*pharmacology
EDAT- 2009/09/02 06:00
MHDA- 2010/01/09 06:00
CRDT- 2009/09/02 09:00
PHST- 2009/07/23 00:00 [received]
PHST- 2009/08/22 00:00 [revised]
PHST- 2009/08/24 00:00 [accepted]
PHST- 2009/09/02 09:00 [entrez]
PHST- 2009/09/02 06:00 [pubmed]
PHST- 2010/01/09 06:00 [medline]
AID - S0027-5107(09)00256-5 [pii]
AID - 10.1016/j.mrfmmm.2009.08.011 [doi]
PST - ppublish
SO  - Mutat Res. 2009 Dec 1;671(1-2):40-4. doi: 10.1016/j.mrfmmm.2009.08.011. Epub 2009 
      Aug 29.