PMID- 19720778
OWN - NLM
STAT- MEDLINE
DCOM- 20090921
LR  - 20240322
IS  - 1942-5546 (Electronic)
IS  - 0025-6196 (Print)
IS  - 0025-6196 (Linking)
VI  - 84
IP  - 9
DP  - 2009 Sep
TI  - Fluorescence in situ hybridization to visualize genetic abnormalities in 
      interphase cells of acinar cell carcinoma, ductal adenocarcinoma, and islet cell 
      carcinoma of the pancreas.
PG  - 801-10
LID - S0025-6196(11)60490-4 [pii]
LID - 10.4065/84.9.801 [doi]
AB  - OBJECTIVE: To use fluorescence in situ hybridization (FISH) to visualize genetic 
      abnormalities in interphase cell nuclei (interphase FISH) of acinar cell 
      carcinoma, ductal adenocarcinoma, and islet cell carcinoma of the pancreas. 
      PATIENTS AND METHODS: Between April 4, 2007, and December 4, 2008, interphase 
      FISH was used to study paraffin-embedded preparations of tissue obtained from 18 
      patients listed in the Mayo Clinic Biospecimen Resource for Pancreas Research 
      with a confirmed diagnosis of acinar cell carcinoma, ductal adenocarcinoma, islet 
      cell carcinoma, or pancreas without evidence of neoplasia. FISH probes were used 
      for chromosome loci of APC (see glossary at end of article for expansion of all 
      gene symbols), BRCA2, CTNNB1, EGFR, ERBB2, CDKN2A, TP53, TYMP, and TYMS. These 
      FISH probes were used with control probes to distinguish among various kinds of 
      chromosome abnormalities of number and structure. RESULTS: FISH abnormalities 
      were observed in 12 (80%) of 15 patients with pancreatic cancer: 5 of 5 patients 
      with acinar cell carcinoma, 5 of 5 patients with ductal adenocarcinoma, and 2 
      (40%) of 5 patients with islet cell carcinoma. All 3 specimens of pancreatic 
      tissue without neoplasia had normal FISH results. Gains of CTNNB1 due to trisomy 
      3 occurred in each tumor with acinar cell carcinoma but in none of the other 
      tumors in this study. FISH abnormalities of all other cancer genes studied were 
      observed in all forms of pancreatic tumors in this investigation. CONCLUSION: 
      FISH abnormalities of CTNNB1 due to trisomy 3 were observed only in acinar cell 
      carcinoma. FISH abnormalities of genes implicated in familial cancer, tumor 
      progression, and the 5-fluorouracil pathway were common but were not associated 
      with specific types of pancreatic cancer.
FAU - Dewald, Gordon W
AU  - Dewald GW
AD  - Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905, USA. 
      gdewald@mayo.edu
FAU - Smyrk, Thomas C
AU  - Smyrk TC
FAU - Thorland, Erik C
AU  - Thorland EC
FAU - McWilliams, Robert R
AU  - McWilliams RR
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
FAU - Keefe, Jeannette G
AU  - Keefe JG
FAU - Belongie, Kimberly J
AU  - Belongie KJ
FAU - Smoley, Stephanie A
AU  - Smoley SA
FAU - Knutson, Darlene L
AU  - Knutson DL
FAU - Fink, Stephanie R
AU  - Fink SR
FAU - Wiktor, Anne E
AU  - Wiktor AE
FAU - Petersen, Gloria M
AU  - Petersen GM
LA  - eng
GR  - P50 CA102701/CA/NCI NIH HHS/United States
GR  - P20 CA102701/CA/NCI NIH HHS/United States
GR  - R01 CA97075/CA/NCI NIH HHS/United States
GR  - P50 CA102701-07/CA/NCI NIH HHS/United States
GR  - R01 CA097075/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Mayo Clin Proc
JT  - Mayo Clinic proceedings
JID - 0405543
SB  - IM
MH  - Carcinoma, Acinar Cell/*genetics/pathology
MH  - Carcinoma, Islet Cell/*genetics/pathology
MH  - Carcinoma, Pancreatic Ductal/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Male
MH  - Pancreatic Neoplasms/*genetics/pathology
PMC - PMC2735430
EDAT- 2009/09/02 06:00
MHDA- 2009/09/22 06:00
PMCR- 2010/03/01
CRDT- 2009/09/02 09:00
PHST- 2009/09/02 09:00 [entrez]
PHST- 2009/09/02 06:00 [pubmed]
PHST- 2009/09/22 06:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - S0025-6196(11)60490-4 [pii]
AID - 0840801 [pii]
AID - 10.4065/84.9.801 [doi]
PST - ppublish
SO  - Mayo Clin Proc. 2009 Sep;84(9):801-10. doi: 10.4065/84.9.801.