PMID- 19722282
OWN - NLM
STAT- MEDLINE
DCOM- 20100708
LR  - 20220227
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 93
IP  - 3
DP  - 2010 Jun 1
TI  - The effects of BMP6 overexpression on adipose stem cell chondrogenesis: 
      Interactions with dexamethasone and exogenous growth factors.
PG  - 994-1003
LID - 10.1002/jbm.a.32589 [doi]
AB  - Adipose-derived stem cells (ASCs) are multipotent progenitors that can be 
      chondrogenically induced by growth factors such as bone morphogenetic protein 6 
      (BMP-6). We hypothesized that nonviral transfection of a BMP-6 construct 
      (pcDNA3-BMP6) would induce chondrogenic differentiation of ASCs encapsulated in 
      alginate beads and that differentiation would be enhanced by the presence of the 
      synthetic glucocorticoid dexamethasone (DEX) or the combination of epidermal 
      growth factor (EGF), fibroblast growth factor-2 (FGF-2), and transforming growth 
      factor beta-1 (TGF-beta1), collectively termed expansion factors (EFs). 
      Chondrogenesis was assessed using quantitative real-time polymerase chain 
      reaction for types I, II, and X collagen, aggrecan, and BMP6. 
      Immunohistochemistry was performed with antibodies for types I, II, and X 
      collagen and chondroitin-4-sulfate. BMP6 overexpression alone induced a moderate 
      chondrogenic response. The inclusion of EFs promoted robust type II collagen 
      expression but also increased type I and X collagen deposition, consistent with a 
      hypertrophic chondrocyte phenotype. Early gene expression data indicated that DEX 
      was synergistic with BMP-6 for chondrogenesis, but immunohistochemistry at 28 
      days showed that DEX reduced glycosaminoglycan accumulation. These results 
      suggest that chondrogenic differentiation of ASCs depends on complex interactions 
      among various growth factors and media supplements, as well as the concentration 
      and duration of growth factor exposure.
FAU - Diekman, Brian O
AU  - Diekman BO
AD  - Orthopaedic Research Laboratories, Department of Surgery, Division of Orthopaedic 
      Surgery, 375 MSRB, Box 3093, Duke University Medical Center, Durham, North 
      Carolina 27710, USA.
FAU - Estes, Bradley T
AU  - Estes BT
FAU - Guilak, Farshid
AU  - Guilak F
LA  - eng
GR  - T32 GM008555/GM/NIGMS NIH HHS/United States
GR  - GM08555/GM/NIGMS NIH HHS/United States
GR  - AG15768/AG/NIA NIH HHS/United States
GR  - R01 AR048852/AR/NIAMS NIH HHS/United States
GR  - R01 AR048182/AR/NIAMS NIH HHS/United States
GR  - AR48852/AR/NIAMS NIH HHS/United States
GR  - RR024128/RR/NCRR NIH HHS/United States
GR  - AR50245/AR/NIAMS NIH HHS/United States
GR  - P01 AR050245/AR/NIAMS NIH HHS/United States
GR  - R01 AG015768/AG/NIA NIH HHS/United States
GR  - UL1 RR024128/RR/NCRR NIH HHS/United States
GR  - AR48182/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Bone Morphogenetic Protein 6)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult
MH  - Bone Morphogenetic Protein 6/genetics/*metabolism
MH  - Cells, Cultured
MH  - Chondrogenesis/*drug effects/genetics
MH  - Chondroitin Sulfates/metabolism
MH  - Collagen/genetics/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Intercellular Signaling Peptides and Proteins/*pharmacology
MH  - Polymerase Chain Reaction
MH  - Stem Cells/*cytology/drug effects/metabolism
PMC - PMC3616877
MID - NIHMS453928
EDAT- 2009/09/02 06:00
MHDA- 2010/07/09 06:00
PMCR- 2013/04/04
CRDT- 2009/09/02 09:00
PHST- 2009/09/02 09:00 [entrez]
PHST- 2009/09/02 06:00 [pubmed]
PHST- 2010/07/09 06:00 [medline]
PHST- 2013/04/04 00:00 [pmc-release]
AID - 10.1002/jbm.a.32589 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Jun 1;93(3):994-1003. doi: 10.1002/jbm.a.32589.