PMID- 19723544
OWN - NLM
STAT- MEDLINE
DCOM- 20091117
LR  - 20090928
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 79
IP  - 1
DP  - 2009 Oct
TI  - Establish a recombinant yeast detection system to study the effect of MIP on 
      transactivation function of hMafF in US2-driven gene transcription.
PG  - 96-100
LID - 10.1016/j.mimet.2009.08.012 [doi]
AB  - The human gene MafF (hMafF) is a member of bZip transcription factor Maf family, 
      but it alone cannot activate its target genes. In 2006, a novel hMafF interacting 
      protein (MIP) was identified. Transient transfection assay in Hela cells 
      suggested that co-expression of MIP and hMafF could activate US2-driven 
      transcription. In this work, we constructed a series of plasmids and transformed 
      YM4271 yeast strain to establish a recombinant yeast detection system. In this 
      system, MIP's expression level could be regulated using glucose incubation or 
      galactose-induced incubation. The expression level of reporter gene LacZ in 
      obtained recombinant yeast strains was measured using quantitative liquid assay. 
      By comparing and analyzing the beta-galactosidase activities of different yeast 
      strains or the same yeast strain in different culture media, the effect of MIP on 
      transactivation driven by nUS2-hMafF was finally determined. Only in the presence 
      of both MIP and hMafF could the nUS2-pLacZi reporter in yeast genome be 
      activated. More importantly, this work established a novel recombinant yeast 
      detection system, which may serve as a powerful tool to study the regulatory 
      mechanisms of transcription complex in the future.
FAU - Ye, Xiaoxia
AU  - Ye X
AD  - Department of Histology and Embryology, Guangdong Medical College, Guangdong 
      Zhanjiang 524023, China. yemissing@yahoo.com.cn
FAU - Shi, Yan
AU  - Shi Y
FAU - Huo, Keke
AU  - Huo K
FAU - Chen, Dong
AU  - Chen D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090831
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Aquaporins)
RN  - 0 (Eye Proteins)
RN  - 0 (MAFF protein, human)
RN  - 0 (MafF Transcription Factor)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (aquaporin 0)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Aquaporins/genetics/*metabolism
MH  - Artificial Gene Fusion
MH  - Eye Proteins/genetics/*metabolism
MH  - Genes, Reporter
MH  - Humans
MH  - MafF Transcription Factor/genetics/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Protein Interaction Mapping/*methods
MH  - Saccharomyces cerevisiae/genetics
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - *Transcriptional Activation
MH  - beta-Galactosidase/genetics/metabolism
EDAT- 2009/09/03 06:00
MHDA- 2009/11/18 06:00
CRDT- 2009/09/03 09:00
PHST- 2009/06/25 00:00 [received]
PHST- 2009/08/20 00:00 [revised]
PHST- 2009/08/20 00:00 [accepted]
PHST- 2009/09/03 09:00 [entrez]
PHST- 2009/09/03 06:00 [pubmed]
PHST- 2009/11/18 06:00 [medline]
AID - S0167-7012(09)00262-0 [pii]
AID - 10.1016/j.mimet.2009.08.012 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2009 Oct;79(1):96-100. doi: 10.1016/j.mimet.2009.08.012. 
      Epub 2009 Aug 31.