PMID- 19724245
OWN - NLM
STAT- MEDLINE
DCOM- 20091028
LR  - 20211020
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 31
DP  - 2009 Sep 1
TI  - Fluorescence in situ hybridization (FISH) protocol in human sperm.
LID - 1405 [pii]
LID - 10.3791/1405 [doi]
AB  - Aneuploidies are the most frequent chromosomal abnormalities in humans. Most of 
      these abnormalities result from meiotic errors during the gametogenic process in 
      the parents. In human males, these errors can lead to the production of 
      spermatozoa with numerical chromosome abnormalities which represent an increased 
      risk of transmitting these anomalies to the offspring. For this reason, the 
      technique of fluorescence in situ hybridization (FISH) on sperm nuclei has become 
      a protocol widely incorporated in the context of clinical diagnosis. This 
      practice provides an estimate of the frequencies of numerical chromosome 
      abnormalities in the gametes of the patients that seek for genetic reproductive 
      advice. To date, the chromosomes most frequently included in sperm FISH analysis 
      are chromosomes X, Y, 13, 18 and 21. This video-article describes, step by step, 
      how to process and fix a human semen sample, how to decondense and denature the 
      sperm chromatin, how to proceed to obtain sperm FISH preparations, and how to 
      visualize the results at the microscope. Special remarks of the most relevant 
      steps are given to achieve the best results.
FAU - Sarrate, Zaida
AU  - Sarrate Z
AD  - Unitat de Biologia Cellular (Facultat de Biociencies).
FAU - Anton, Ester
AU  - Anton E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20090901
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - *Chromosomes, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Spermatozoa/chemistry/*ultrastructure
MH  - Tissue Fixation/methods
PMC - PMC3150054
EDAT- 2009/09/03 06:00
MHDA- 2009/10/29 06:00
PMCR- 2011/09/01
CRDT- 2009/09/03 09:00
PHST- 2009/09/03 09:00 [entrez]
PHST- 2009/09/03 06:00 [pubmed]
PHST- 2009/10/29 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - 1405 [pii]
AID - 10.3791/1405 [doi]
PST - epublish
SO  - J Vis Exp. 2009 Sep 1;(31):1405. doi: 10.3791/1405.