PMID- 19724856
OWN - NLM
STAT- MEDLINE
DCOM- 20091109
LR  - 20190606
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 22
IP  - 4
DP  - 2009 Oct
TI  - Effect of CD133/prominin-1 antisense oligodeoxynucleotide on in vitro growth 
      characteristics of Huh-7 human hepatocarcinoma cells and U251 human glioma cells.
PG  - 781-7
AB  - The stem cell marker CD133/prominin-1, is a pentaspan membrane glycoprotein, 
      which has also been identified as a cancer stem cell (CSC) marker in several 
      solid tumor types, including those of the prostate, liver, colon and brain. The 
      function of CD133 in these tumors is still unknown. We hypothesize that the 
      function of CD133 is correlated with the characteristics of the CD133+ CSCs and 
      may affect the growth of the tumor cell population as a whole. In this study we 
      used antisense oligodeoxynucleotides (ASODNs) of CD133 to knock-down (KD) CD133 
      expression. We showed that the CD133-KD inhibited proliferation of U251 human 
      glioma cells, decreased the colony forming ability and altered the cell cycle 
      distribution in Huh-7 human hepatocellular carcinoma cells. Our data suggest that 
      CD133 may play an important functional role in the growth of these tumor cells. 
      Moreover, this study also showed that prominin-2, another protein in the same 
      family as CD133/prominin-1, may not have a similar function.
FAU - Yao, Jie
AU  - Yao J
AD  - Cancer Center, Union Hospital, Huazhong University of Sciences and Technology, 
      Wuhan 430023, Hubei Province, P.R. China.
FAU - Zhang, Tao
AU  - Zhang T
FAU - Ren, Jinghua
AU  - Ren J
FAU - Yu, Minxia
AU  - Yu M
FAU - Wu, Gang
AU  - Wu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (PROM1 protein, human)
RN  - 0 (PROM2 protein, human)
RN  - 0 (Peptides)
SB  - IM
MH  - AC133 Antigen
MH  - Antigens, CD/genetics/*metabolism
MH  - Brain Neoplasms/genetics/metabolism/pathology
MH  - Carcinoma, Hepatocellular/genetics/*metabolism/pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Glioma/genetics/*metabolism/pathology
MH  - Glycoproteins/genetics/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Liver Neoplasms/genetics/metabolism/pathology
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Oligodeoxyribonucleotides, Antisense
MH  - Peptides/genetics/*metabolism
MH  - Polymerase Chain Reaction
MH  - Transfection
EDAT- 2009/09/03 06:00
MHDA- 2009/11/10 06:00
CRDT- 2009/09/03 09:00
PHST- 2009/09/03 09:00 [entrez]
PHST- 2009/09/03 06:00 [pubmed]
PHST- 2009/11/10 06:00 [medline]
AID - 10.3892/or_00000500 [doi]
PST - ppublish
SO  - Oncol Rep. 2009 Oct;22(4):781-7. doi: 10.3892/or_00000500.