PMID- 19724880
OWN - NLM
STAT- MEDLINE
DCOM- 20091214
LR  - 20190606
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 24
IP  - 4
DP  - 2009 Oct
TI  - Identification of sex hormone binding globulin-interacting proteins in the brain 
      using phage display screening.
PG  - 421-6
AB  - The present study reports the identification of human sex hormone binding 
      globulin (SHBG)-interacting proteins in the brain using a phage display-based 
      screening technology. Phage display is a system in which a foreign protein is 
      displayed on the surface of a bacteriophage as a fusion protein with one of the 
      coat proteins of the bacteriophage. T7 phage clones expressing normal human brain 
      proteins (human normal brain phage-display cDNA expression library) were screened 
      using SHBG as bait. The bound phage clones were then identified by DNA sequencing 
      and by BLAST search analysis. Of the twenty binding proteins analyzed, three were 
      found to be membrane-associated proteins: synaptosomal associated protein 25 
      (SNAP25), Thy-1 cell surface antigen and zonadhesin. Further studies will 
      determine if the interactions of SHBG with these proteins have any role in the 
      internalization and cell signaling events or whether they contribute to steroid 
      delivery to specific cells.
FAU - Gnanasekar, M
AU  - Gnanasekar M
AD  - Department of Biomedical Sciences, University of Illinois College of Medicine, 
      Rockford, IL 61107, USA. mgnanas@uic.edu
FAU - Suleman, F G
AU  - Suleman FG
FAU - Ramaswamy, K
AU  - Ramaswamy K
FAU - Caldwell, J D
AU  - Caldwell JD
LA  - eng
GR  - R21-MH069810/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Library)
RN  - 0 (Proteins)
RN  - 0 (Sex Hormone-Binding Globulin)
RN  - 0 (Synaptosomal-Associated Protein 25)
RN  - 0 (Thy-1 Antigens)
RN  - 0 (zonadhesin)
SB  - IM
MH  - Bacteriophage T7/genetics/metabolism
MH  - Brain/*metabolism
MH  - Humans
MH  - Membrane Proteins/genetics/metabolism
MH  - *Peptide Library
MH  - Polymerase Chain Reaction
MH  - Protein Binding/genetics
MH  - Proteins/genetics/*metabolism
MH  - Sex Hormone-Binding Globulin/*metabolism
MH  - Synaptosomal-Associated Protein 25/genetics/metabolism
MH  - Thy-1 Antigens/genetics/metabolism
EDAT- 2009/09/03 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/03 09:00
PHST- 2009/09/03 09:00 [entrez]
PHST- 2009/09/03 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.3892/ijmm_00000248 [doi]
PST - ppublish
SO  - Int J Mol Med. 2009 Oct;24(4):421-6. doi: 10.3892/ijmm_00000248.