PMID- 19725068
OWN - NLM
STAT- MEDLINE
DCOM- 20100330
LR  - 20131121
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 73
IP  - 3
DP  - 2010 Mar
TI  - The olfactory organ of the trout Salmo trutta fario: a novel localization for a 
      progestin receptor.
PG  - 206-14
LID - 10.1002/jemt.20776 [doi]
AB  - A progestin receptor (PR) has been detected in the olfactory organ of the trout 
      Salmo trutta fario. The specificity of this receptor was high for 
      17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-DP), but it also bound 
      17alpha-hydroxy-progesterone (17alpha-OHP) and 21-hydroxyprogesterone (21-OHP), 
      even when present at low concentrations (10-fold in relative binding affinity 
      assay). Progesterone (P) competed effectively at much higher concentrations 
      (1,000-fold in relative binding affinity assay). Immunohistochemical studies 
      carried out with three different monoclonal antibodies against human progesterone 
      receptor (hPR), chicken progesterone receptor hinge region (cPR), and chicken 
      progesterone receptor A/B domain (PR22), revealed that immunoreactivity was 
      present in the epithelium of the olfactory organ of females and males of the 
      trout Salmo trutta fario only against hPR. Western blotting showed two hPR 
      immunoreactive bands of about 62 and 66 kDa. Finally, a portion of the cDNA of 
      about 300 nucleotides extending over the DNA binding domain and the ligand 
      binding domain was cloned and sequenced, revealing a high degree of sequence 
      homology of the PR in Salmo trutta fario with the PR in other teleosts.
FAU - Varricchio, Ettore
AU  - Varricchio E
AD  - Department of Biological and Environmental Sciences, University of Sannio, Via 
      Port'Arsa, 11, Benevento 82100, Italy.
FAU - Coccia, Elena
AU  - Coccia E
FAU - Putti, Rosalba
AU  - Putti R
FAU - Paolucci, Marina
AU  - Paolucci M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA, Complementary)
RN  - 0 (Hydroxyprogesterones)
RN  - 0 (Receptors, Progesterone)
RN  - 10456-50-5 (17,20-dihydroxy-4-pregnen-3-one)
RN  - 40GP35YQ49 (Desoxycorticosterone)
RN  - 68-96-2 (17-alpha-Hydroxyprogesterone)
SB  - IM
MH  - 17-alpha-Hydroxyprogesterone/metabolism
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Blotting, Western
MH  - Chickens
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Desoxycorticosterone/metabolism
MH  - Female
MH  - Humans
MH  - Hydroxyprogesterones/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Molecular Weight
MH  - Olfactory Pathways/*chemistry/physiology
MH  - Protein Binding
MH  - Receptors, Progesterone/*analysis/chemistry/genetics/metabolism
MH  - Salmonidae/genetics/*physiology
MH  - Sequence Analysis, DNA
EDAT- 2009/09/03 06:00
MHDA- 2010/03/31 06:00
CRDT- 2009/09/03 09:00
PHST- 2009/09/03 09:00 [entrez]
PHST- 2009/09/03 06:00 [pubmed]
PHST- 2010/03/31 06:00 [medline]
AID - 10.1002/jemt.20776 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2010 Mar;73(3):206-14. doi: 10.1002/jemt.20776.