PMID- 1972666
OWN - NLM
STAT- MEDLINE
DCOM- 19900731
LR  - 20190828
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 53
IP  - 2
DP  - 1990 May 1
TI  - Prenatal development of mesencephalic and diencephalic dopaminergic systems in 
      the male and female rat.
PG  - 222-9
AB  - Previous results had suggested mesencephalic dopaminergic neurons in cultures of 
      gestational day (GD) 14 rat embryonic brains to be characterized by an early 
      maturation and acquisition of sex-related differences in transmitter uptake. 
      Therefore development of dopaminergic systems was reexamined in the rat in vivo 
      with special emphasis on the prenatal period, mesencephalo-hypothalamic 
      relationships, and possible sex differences. Perfusion-fixed brains of GD 14, 17, 
      20, 21 and newborn rats were sectioned or processed as whole-mounts and 
      immunostained for tyrosine hydroxylase (TH). Total numbers of mesencephalic 
      TH-immunoreactive cell bodies as assessed by a stereological method rose between 
      GD 14 and 17 and fell again between GD 17 and 21. As early as GD 14, a prominent 
      mesencephalo-hypothalamic projection was observed coming off the medial forebrain 
      bundle and terminating in the retrochiasmatic region. Two additional 
      TH-immunoreactive fiber bundles leaving the medial forebrain bundle, one rostral 
      and one caudal to the former, and terminating in the paraventricular and 
      premammillary region, respectively, were noticed on GD 17. Careful examination of 
      developing TH-immunoreactive neurons in the lower brainstem ascertained that 
      there was no interference from ascending catecholaminergic fibers other than 
      dopaminergic of mesencephalic origin during this early prenatal period. All 3 
      mesencephalo-hypothalamic projections had largely disappeared by GD 20 and were 
      no longer detectable as distinct fiber bundles thereafter. There were subtle sex 
      differences in numbers and distribution of both mesencephalic and diencephalic 
      TH-immunoreactive neurons present at GD 17, which thus occurred prior to 
      manifestation of other well-known sexual dimorphisms of the rat brain.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Reisert, I
AU  - Reisert I
AD  - Abteilung Anatomie und Zellbiologie, Universitat Ulm, F.R.G.
FAU - Schuster, R
AU  - Schuster R
FAU - Zienecker, R
AU  - Zienecker R
FAU - Pilgrim, C
AU  - Pilgrim C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Diencephalon/*embryology/metabolism
MH  - Dopamine/*metabolism
MH  - *Embryonic and Fetal Development
MH  - Female
MH  - Immunohistochemistry
MH  - Male
MH  - Mesencephalon/*embryology/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - *Sex Characteristics
MH  - Tyrosine 3-Monooxygenase/*metabolism
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
AID - 0165-3806(90)90010-V [pii]
AID - 10.1016/0165-3806(90)90010-v [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1990 May 1;53(2):222-9. doi: 
      10.1016/0165-3806(90)90010-v.