PMID- 19729031
OWN - NLM
STAT- MEDLINE
DCOM- 20091202
LR  - 20211020
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 241
IP  - 3
DP  - 2009 Dec 15
TI  - Pulmonary effects of inhaled diesel exhaust in aged mice.
PG  - 283-93
LID - 10.1016/j.taap.2009.08.025 [doi]
AB  - Pulmonary morbidity and mortality resulting from exposure to fine particulate 
      matter (PM) increases with age. The present studies analyzed potential mechanisms 
      underlying increased susceptibility of the elderly to PM using diesel exhaust 
      (DE) as a model. Mice (2 m and 18 m) were exposed to DE (0, 300, and 1000 
      microg/m(3)) for 3 h once (single) or 3 h/day for 3 days (repeated). 
      Bronchoalveolar lavage fluid (BAL), serum and lung tissue were collected 0 and 24 
      h later. Exposure to DE resulted in structural alterations in the lungs of older 
      but not younger mice, including patchy thickening of the alveolar septa and 
      inflammatory cell localization in alveolar spaces. These effects were most 
      pronounced 24 h after a single exposure to the higher dose of DE. Significant 
      increases in BAL nitrogen oxides were also noted in older mice, as well as 
      expression of lipocalin 24p3, an oxidative stress marker in the lung with no 
      effects in younger mice. Following DE inhalation, expression of Tumor Necrosis 
      Factor alpha (TNFalpha) was upregulated in lungs of both younger and older mice; 
      however, this was attenuated in older animals. Whereas exposure to DE resulted in 
      increases in lung Interleukin-6 (IL-6) expression in both older and younger mice, 
      IL-8 increased only in older animals. In younger mice, constitutive expression of 
      manganese superoxide dismutase (MnSOD) decreased after DE exposure, while in 
      older mice, constitutive MnSOD was not detectable and DE had no effect on 
      expression of this antioxidant. Taken together, these results suggest that 
      altered generation of inflammatory mediators and MnSOD may contribute to 
      increased susceptibility of older mice to inhaled DE.
FAU - Sunil, Vasanthi R
AU  - Sunil VR
AD  - Department of Pharmacology and Toxicology, Rutgers University, Ernest Mario 
      School of Pharmacy, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA. 
      sunilvr@eohsi.rutgers.edu
FAU - Patel, Kinal J
AU  - Patel KJ
FAU - Mainelis, Gediminas
AU  - Mainelis G
FAU - Turpin, Barbara J
AU  - Turpin BJ
FAU - Ridgely, Sherritta
AU  - Ridgely S
FAU - Laumbach, Robert J
AU  - Laumbach RJ
FAU - Kipen, Howard M
AU  - Kipen HM
FAU - Nazarenko, Yevgen
AU  - Nazarenko Y
FAU - Veleeparambil, Manoj
AU  - Veleeparambil M
FAU - Gow, Andrew J
AU  - Gow AJ
FAU - Laskin, Jeffrey D
AU  - Laskin JD
FAU - Laskin, Debra L
AU  - Laskin DL
LA  - eng
GR  - U54 AR055073-03/AR/NIAMS NIH HHS/United States
GR  - R01 CA132624/CA/NCI NIH HHS/United States
GR  - R01 CA132624-02/CA/NCI NIH HHS/United States
GR  - U54 AR055073-02/AR/NIAMS NIH HHS/United States
GR  - ES013520/ES/NIEHS NIH HHS/United States
GR  - R01 GM034310/GM/NIGMS NIH HHS/United States
GR  - U54 AR055073/AR/NIAMS NIH HHS/United States
GR  - ES005022/ES/NIEHS NIH HHS/United States
GR  - K08 ES013520-04/ES/NIEHS NIH HHS/United States
GR  - GM034310/GM/NIGMS NIH HHS/United States
GR  - CA132624/CA/NCI NIH HHS/United States
GR  - R01 HL086621/HL/NHLBI NIH HHS/United States
GR  - R01 GM034310-24/GM/NIGMS NIH HHS/United States
GR  - AR055073/AR/NIAMS NIH HHS/United States
GR  - K08 ES013520-02/ES/NIEHS NIH HHS/United States
GR  - U54 AR055073-04/AR/NIAMS NIH HHS/United States
GR  - R01 ES004738-10/ES/NIEHS NIH HHS/United States
GR  - P30 ES005022/ES/NIEHS NIH HHS/United States
GR  - P30 ES005022-20/ES/NIEHS NIH HHS/United States
GR  - P30 ES005022-21/ES/NIEHS NIH HHS/United States
GR  - R01 CA132624-01/CA/NCI NIH HHS/United States
GR  - R01 ES004738-14/ES/NIEHS NIH HHS/United States
GR  - ES004738/ES/NIEHS NIH HHS/United States
GR  - K08 ES013520/ES/NIEHS NIH HHS/United States
GR  - R01 GM034310-23/GM/NIGMS NIH HHS/United States
GR  - R01 ES004738/ES/NIEHS NIH HHS/United States
GR  - K08 ES013520-03/ES/NIEHS NIH HHS/United States
GR  - U54 AR055073-01/AR/NIAMS NIH HHS/United States
GR  - R01 ES004738-15/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090901
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Aerosols)
RN  - 0 (Antioxidants)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Nitrogen Oxides)
RN  - 0 (Particulate Matter)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vehicle Emissions)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Aerosols
MH  - Aging/*physiology
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Immunohistochemistry
MH  - Inflammation Mediators/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lung/drug effects/metabolism/pathology
MH  - Lung Diseases/*chemically induced/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Nitrogen Oxides/metabolism
MH  - Oxidative Stress/drug effects
MH  - Particle Size
MH  - Particulate Matter/analysis/toxicity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Superoxide Dismutase/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vehicle Emissions/*toxicity
PMC - PMC3102559
MID - NIHMS210916
EDAT- 2009/09/05 06:00
MHDA- 2009/12/16 06:00
PMCR- 2011/05/26
CRDT- 2009/09/05 06:00
PHST- 2009/05/06 00:00 [received]
PHST- 2009/08/20 00:00 [revised]
PHST- 2009/08/23 00:00 [accepted]
PHST- 2009/09/05 06:00 [entrez]
PHST- 2009/09/05 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2011/05/26 00:00 [pmc-release]
AID - S0041-008X(09)00369-X [pii]
AID - 10.1016/j.taap.2009.08.025 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2009 Dec 15;241(3):283-93. doi: 
      10.1016/j.taap.2009.08.025. Epub 2009 Sep 1.