PMID- 19729194
OWN - NLM
STAT- MEDLINE
DCOM- 20100114
LR  - 20141120
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 72
IP  - 8
DP  - 2009 Nov
TI  - Amphiregulin promotes the proliferation of trophoblast cells during 
      preimplantation development of porcine embryos.
PG  - 1023-31
LID - 10.1016/j.theriogenology.2009.05.021 [doi]
AB  - Our objective was to investigate the effects of in vitro culture (IVC) medium 
      supplemented with amphiregulin (AREG) on the preimplantation embryonic 
      development of porcine (Genus: Sus domestica, Species: Landrace) embryos derived 
      from in vitro fertilization (IVF) and parthenogenetic activation (PA). In vitro 
      fertilization and PA embryos at the 1-cell stage were cultured in IVC medium 
      supplemented with 0, 0.5, 5, or 50 ng/mL AREG for 7 d. There were significantly 
      greater total numbers of cells in blastocysts of IVF and PA embryos cultured with 
      50 ng/mL AREG compared with that of controls. In vitro fertilization and PA 
      embryos were then cultured in NCSU-23 medium supplemented with 50 ng/mL AREG on 
      Days 1 through 7, Days 1 through 3 (early stage), or Days 4 through 7 (late 
      stage), or without AREG. There were significantly greater numbers of trophoblast 
      cells in the late-stage and full-term groups of IVF and PA embryos than in the 
      early-stage and control groups. The presence of AREG protein in IVF-derived 
      blastocysts was detected using a polyclonal AREG antibody and indirect 
      immunofluorescence. Amphiregulin protein was localized in both the cytoplasm and 
      nucleus. Using real-time polymerase chain reaction, we detected the expression of 
      AREG mRNA in all developmental stages of IVF and PA embryos; however, the 
      expression level varied according to stage. Thus, the incubation of porcine IVF 
      and PA embryos in AREG-supplemented culture medium mainly at the late 
      preimplantation stage increases the numbers of trophoblast cells.
FAU - Lee, J H
AU  - Lee JH
AD  - College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, 
      Korea.
FAU - Lee, E
AU  - Lee E
FAU - Biswas, D
AU  - Biswas D
FAU - Jeung, C S
AU  - Jeung CS
FAU - Lee, G S
AU  - Lee GS
FAU - Hyun, S H
AU  - Hyun SH
FAU - Jeung, E B
AU  - Jeung EB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090903
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Amphiregulin)
RN  - 0 (Culture Media)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Culture Media
MH  - Cytoplasm/metabolism
MH  - *Embryo Culture Techniques
MH  - Embryonic Development/*drug effects
MH  - Fertilization in Vitro
MH  - Glycoproteins/analysis/metabolism/*pharmacology
MH  - Intercellular Signaling Peptides and Proteins/analysis/metabolism/*pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - *Swine
MH  - Trophoblasts/*drug effects/metabolism
EDAT- 2009/09/05 06:00
MHDA- 2010/01/15 06:00
CRDT- 2009/09/05 06:00
PHST- 2009/01/06 00:00 [received]
PHST- 2009/05/24 00:00 [revised]
PHST- 2009/05/28 00:00 [accepted]
PHST- 2009/09/05 06:00 [entrez]
PHST- 2009/09/05 06:00 [pubmed]
PHST- 2010/01/15 06:00 [medline]
AID - S0093-691X(09)00305-7 [pii]
AID - 10.1016/j.theriogenology.2009.05.021 [doi]
PST - ppublish
SO  - Theriogenology. 2009 Nov;72(8):1023-31. doi: 
      10.1016/j.theriogenology.2009.05.021. Epub 2009 Sep 3.