PMID- 19729666
OWN - NLM
STAT- MEDLINE
DCOM- 20100104
LR  - 20211020
IS  - 1535-4970 (Electronic)
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 180
IP  - 12
DP  - 2009 Dec 15
TI  - Lung dendritic cell expression of maturation molecules increases with worsening 
      chronic obstructive pulmonary disease.
PG  - 1179-88
LID - 10.1164/rccm.200904-0552OC [doi]
AB  - RATIONALE: Dendritic cells (DCs) have not been well studied in chronic 
      obstructive pulmonary disease (COPD), yet their integral role in activating and 
      differentiating T cells makes them potential participants in COPD pathogenesis. 
      OBJECTIVES: To determine the expression of maturation molecules by individual DC 
      subsets in relationship to COPD stage and to expression of the acute activation 
      marker CD69 by lung CD4(+) T cells. METHODS: We nonenzymatically released lung 
      leukocytes from human surgical specimens (n = 42) and used flow cytometry to 
      identify three DC subsets (mDC1, mDC2, and pDC) and to measure their expression 
      of three costimulatory molecules (CD40, CD80 and CD86) and of CD83, the 
      definitive marker of DC maturation. Spearman nonparametric correlation analysis 
      was used to identify significant correlations between expression of DC maturation 
      molecules and COPD severity. MEASUREMENTS AND MAIN RESULTS: Expression of CD40 by 
      mDC1 and mDC2 and of CD86 by mDC2 was high regardless of GOLD stage, but CD80 and 
      CD83 on these two DC subsets increased with disease progression. pDC also showed 
      significant increases in expression of CD40 and CD80. Expression of all but one 
      of the DC molecules that increased with COPD severity also correlated with CD69 
      expression on lung CD4(+) T cells from the same patients, with the exception of 
      CD83 on mDC2. CONCLUSIONS: This cross-sectional study implies that COPD 
      progression is associated with significant increases in costimulatory molecule 
      expression by multiple lung DC subsets. Interactions with lung DCs may contribute 
      to the immunophenotype of CD4(+) T cells in advanced COPD. Clinical trial 
      registered with www.clinicaltrials.gov (NCT00281229).
FAU - Freeman, Christine M
AU  - Freeman CM
AD  - Pulmonary and Critical Care Medicine Section, VA Ann Arbor Healthcare System, Ann 
      Arbor, MI, USA.
FAU - Martinez, Fernando J
AU  - Martinez FJ
FAU - Han, MeiLan K
AU  - Han MK
FAU - Ames, Theresa M
AU  - Ames TM
FAU - Chensue, Stephen W
AU  - Chensue SW
FAU - Todt, Jill C
AU  - Todt JC
FAU - Arenberg, Douglas A
AU  - Arenberg DA
FAU - Meldrum, Catherine A
AU  - Meldrum CA
FAU - Getty, Christi
AU  - Getty C
FAU - McCloskey, Lisa
AU  - McCloskey L
FAU - Curtis, Jeffrey L
AU  - Curtis JL
LA  - eng
SI  - ClinicalTrials.gov/NCT00281229
GR  - R01 CA094121-05/CA/NCI NIH HHS/United States
GR  - P30 CA46952/CA/NCI NIH HHS/United States
GR  - K24HL04212/HL/NHLBI NIH HHS/United States
GR  - R01 CA094121/CA/NCI NIH HHS/United States
GR  - R01 HL082480/HL/NHLBI NIH HHS/United States
GR  - KL2 RR024987/RR/NCRR NIH HHS/United States
GR  - K24 HL004212/HL/NHLBI NIH HHS/United States
GR  - N01 HR046162/HR/NHLBI NIH HHS/United States
GR  - T32 HL07749/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090903
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Lectins, C-Type)
SB  - IM
CIN - Am J Respir Crit Care Med. 2009 Dec 15;180(12):1166-7. PMID: 19949238
MH  - Aged
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, T-Lymphocyte/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/immunology
MH  - Cross-Sectional Studies
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Lectins, C-Type
MH  - Lung/*immunology
MH  - Male
MH  - Middle Aged
MH  - Pulmonary Disease, Chronic Obstructive/*immunology
MH  - Severity of Illness Index
MH  - Smoking/immunology
PMC - PMC2796731
EDAT- 2009/09/05 06:00
MHDA- 2010/01/05 06:00
PMCR- 2010/12/15
CRDT- 2009/09/05 06:00
PHST- 2009/09/05 06:00 [entrez]
PHST- 2009/09/05 06:00 [pubmed]
PHST- 2010/01/05 06:00 [medline]
PHST- 2010/12/15 00:00 [pmc-release]
AID - 200904-0552OC [pii]
AID - ajrccm180121179 [pii]
AID - 10.1164/rccm.200904-0552OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2009 Dec 15;180(12):1179-88. doi: 
      10.1164/rccm.200904-0552OC. Epub 2009 Sep 3.