PMID- 19732142
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20091006
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 57
IP  - 2
DP  - 2009 Nov
TI  - Cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a 
      domestic porcine breed found in Henan.
PG  - 129-35
LID - 10.1111/j.1574-695X.2009.00589.x [doi]
AB  - To evaluate the effects of recombinant porcine interleukin-18 (rpIL-18) on the 
      replication of viruses in host cells and proliferation of lymphocytes, porcine 
      IL-18 (pIL-18) isolated from a domestic big-white porcine breed found in the 
      Henan province (HN) was cloned using a reverse transcriptase-PCR. The cloned HN 
      pIL-18 contained an ORF of 579 base pairs encoding a 192-amino-acid precursor 
      protein. The amino acid sequence of HN pIL-18 was compared with all the other 
      pIL-18 amino acid sequences and varied by at least one amino acid to the 
      consensus of all the others available. HN pIL-18 mature protein gene was inserted 
      into a prokaryotic vector pGEX-4T-1 and expressed in Escherichia coli BL21. The 
      expression of glutathione-S-transferase-pIL18 fusion protein was confirmed by 
      sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot 
      analysis. The rpIL-18 induced in vitro proliferation of concanavalin-A-stimulated 
      porcine splenocytes, as revealed by the MTT assay. We studied the antiviral 
      activities of the rpIL-18 on the replication of porcine reproductive and 
      respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), and porcine 
      parvovirus (PPV) cultured in two homologous cell lines. The results suggested 
      that rpIL-18 can stimulate the proliferation of lymphocytes and inhibit viral 
      pathogens infecting the porcine population.
FAU - Chen, Hong-Ying
AU  - Chen HY
AD  - Henan Agricultural University, Zhengzhou, China.
FAU - Zheng, Lan-Lan
AU  - Zheng LL
FAU - Li, Xin-Sheng
AU  - Li XS
FAU - Wei, Zhan-Yong
AU  - Wei ZY
FAU - Cui, Bao-An
AU  - Cui BA
FAU - Li, Xiao-Kang
AU  - Li XK
FAU - Liu, Jin-Peng
AU  - Liu JP
FAU - Yin, Hong-Zheng
AU  - Yin HZ
FAU - Meng, Jiang-Tao
AU  - Meng JT
FAU - Zhang, Yan
AU  - Zhang Y
FAU - Li, Shi-Min
AU  - Li SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090727
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Cell Growth Processes/drug effects
MH  - Cell Line
MH  - China
MH  - Cloning, Molecular/methods
MH  - Cytopathogenic Effect, Viral
MH  - Escherichia coli/genetics/metabolism
MH  - Herpesvirus 1, Suid/physiology
MH  - Interleukin-18/biosynthesis/*genetics/isolation & purification/*pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocytes/drug effects/immunology
MH  - Parvovirus, Porcine/physiology
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - Recombinant Proteins/biosynthesis/genetics/isolation & purification/pharmacology
MH  - Sequence Analysis, Protein
MH  - Swine
MH  - Virus Replication/drug effects
EDAT- 2009/09/08 06:00
MHDA- 2010/02/02 06:00
CRDT- 2009/09/08 06:00
PHST- 2009/09/08 06:00 [entrez]
PHST- 2009/09/08 06:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
AID - FIM589 [pii]
AID - 10.1111/j.1574-695X.2009.00589.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2009 Nov;57(2):129-35. doi: 
      10.1111/j.1574-695X.2009.00589.x. Epub 2009 Jul 27.