PMID- 19733743
OWN - NLM
STAT- MEDLINE
DCOM- 20091217
LR  - 20211020
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Print)
IS  - 0003-2670 (Linking)
VI  - 651
IP  - 1
DP  - 2009 Sep 28
TI  - Affinity-based microdialysis sampling using heparin for in vitro collection of 
      human cytokines.
PG  - 105-11
LID - 10.1016/j.aca.2009.08.009 [doi]
AB  - Microdialysis sampling is a widely used method to sample from complex biological 
      matrices. Cytokines are important signaling proteins that are typically recovered 
      with low relative recovery values during microdialysis sampling. Heparin was 
      included in the microdialysis perfusion fluid as an affinity agent to increase in 
      vitro recovery of different cytokines through polyethersulfone (PES) 
      microdialysis membranes with 100 kDa molecular weight cutoff. No change in fluid 
      volumes collected from the microdialysis probes occurred when heparin was 
      included in the perfusion fluid up to concentrations of 10 microM. The loss of 
      heparin (10 microM) across the dialysis membrane was minimal (2.7+/-0.9%, n=3). 
      Additionally, heparin at these concentrations did not interfere with the cytokine 
      immunoassays. The control and heparin-enhanced relative recoveries for five human 
      cytokines using 0.1 microM heparin in the microdialysis perfusion fluid flowing 
      at 0.5 microL min(-1) were (n=3): interleukin-4 (IL-4), 4.2+/-0.5% and 
      7.2+/-3.1%; interleukin-6 (IL-6), 1.4+/-0.8% and 3.6+/-1.3%; interleukin-7 
      (IL-7), 1.3+/-0.8% and 4.8+/-1.8%; monocyte chemoattractant protein-1 (MCP-1), 
      9.0+/-1.6% and 19.5+/-2.7%; and tumor necrosis factor-alpha (TNF-alpha), 
      7.4+/-1.3% and 16.9+/-1.6%, respectively. Heparin increased the microdialysis 
      sampling relative recovery of several human cytokines in vitro.
FAU - Wang, Yuexi
AU  - Wang Y
AD  - Department of Chemistry and Chemical Biology, Center for Biotechnology and 
      Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, 
      USA.
FAU - Stenken, Julie A
AU  - Stenken JA
LA  - eng
GR  - R01 EB001441/EB/NIBIB NIH HHS/United States
GR  - R01 EB001441-04/EB/NIBIB NIH HHS/United States
GR  - R01 EB001441-05A1/EB/NIBIB NIH HHS/United States
GR  - EB001441/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090814
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-7)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Chemokine CCL2/isolation & purification
MH  - Cytokines/*isolation & purification
MH  - Heparin/*chemistry
MH  - Humans
MH  - Interleukin-4/isolation & purification
MH  - Interleukin-6/isolation & purification
MH  - Interleukin-7/isolation & purification
MH  - Microdialysis/*methods
MH  - Tumor Necrosis Factor-alpha/isolation & purification
MH  - Ultrafiltration
PMC - PMC2778253
MID - NIHMS145832
EDAT- 2009/09/08 06:00
MHDA- 2009/12/18 06:00
PMCR- 2010/09/28
CRDT- 2009/09/08 06:00
PHST- 2009/06/18 00:00 [received]
PHST- 2009/07/30 00:00 [revised]
PHST- 2009/08/11 00:00 [accepted]
PHST- 2009/09/08 06:00 [entrez]
PHST- 2009/09/08 06:00 [pubmed]
PHST- 2009/12/18 06:00 [medline]
PHST- 2010/09/28 00:00 [pmc-release]
AID - S0003-2670(09)01051-4 [pii]
AID - 10.1016/j.aca.2009.08.009 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2009 Sep 28;651(1):105-11. doi: 10.1016/j.aca.2009.08.009. Epub 
      2009 Aug 14.