PMID- 19734213
OWN - NLM
STAT- MEDLINE
DCOM- 20091116
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 183
IP  - 7
DP  - 2009 Oct 1
TI  - The ssRNA genome of human rhinovirus induces a type I IFN response but fails to 
      induce maturation in human monocyte-derived dendritic cells.
PG  - 4440-8
LID - 10.4049/jimmunol.0804147 [doi]
AB  - Dendritic cells (DCs) use pattern recognition receptors to sense invading viruses 
      and triggering of these receptors induces a maturation program. Human 
      rhinoviruses (HRVs) belong to the family of Picornaviridae, which have a 
      single-stranded, coding RNA genome. Because HRV does not replicate in DCs, we 
      used genomic RNA from HRV in this study to analyze the impact of natural 
      occurring viral ssRNA on DC function. We found that transfection of human 
      monocyte-derived DCs with viral ssRNA induced type I IFN production but failed to 
      activate the NF-kappaB pathway in DCs. In line with this observation, the 
      up-regulation of typical maturation markers such as CD83 or the production of the 
      proinflammatory cytokines IL-12p40, IL-6, and TNF-alpha was not detectable. Most 
      importantly, the T cell stimulatory capacity of viral ssRNA-treated DCs was not 
      enhanced and remained at the level of immature DCs. Taken together, our results 
      demonstrate that viral ssRNA efficiently activates the innate defense arm of DCs, 
      whereas it is insufficient to activate the stimulatory capacity of DCs for the 
      adaptive defense responses.
FAU - Schrauf, Catharina
AU  - Schrauf C
AD  - Institute of Immunology, Medical University of Vienna, Vienna 1090, Austria.
FAU - Kirchberger, Stefanie
AU  - Kirchberger S
FAU - Majdic, Otto
AU  - Majdic O
FAU - Seyerl, Maria
AU  - Seyerl M
FAU - Zlabinger, Gerhard-Johann
AU  - Zlabinger GJ
FAU - Stuhlmeier, Karl Manfred
AU  - Stuhlmeier KM
FAU - Sachet, Monika
AU  - Sachet M
FAU - Seipelt, Joachim
AU  - Seipelt J
FAU - Stockl, Johannes
AU  - Stockl J
LA  - eng
GR  - P 20266/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090904
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Biomarkers)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interferon Type I)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/genetics/*immunology
MH  - Cell Line
MH  - Dendritic Cells/*immunology/pathology/virology
MH  - Down-Regulation/genetics/*immunology
MH  - Genome, Viral/*immunology
MH  - Growth Inhibitors/genetics/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Immunity, Innate/genetics
MH  - Immunophenotyping
MH  - Inflammation Mediators/metabolism
MH  - Interferon Type I/*biosynthesis/genetics
MH  - Lymphocyte Activation/genetics
MH  - Monocytes/*immunology/pathology/virology
MH  - RNA, Viral/antagonists & inhibitors/*immunology/metabolism
MH  - Rhinovirus/genetics/*immunology
MH  - Virus Replication/genetics/immunology
EDAT- 2009/09/08 06:00
MHDA- 2009/11/17 06:00
CRDT- 2009/09/08 06:00
PHST- 2009/09/08 06:00 [entrez]
PHST- 2009/09/08 06:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - jimmunol.0804147 [pii]
AID - 10.4049/jimmunol.0804147 [doi]
PST - ppublish
SO  - J Immunol. 2009 Oct 1;183(7):4440-8. doi: 10.4049/jimmunol.0804147. Epub 2009 Sep 
      4.