PMID- 19734680
OWN - NLM
STAT- MEDLINE
DCOM- 20091231
LR  - 20211203
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 73
IP  - 9
DP  - 2009 Sep
TI  - Ethionine-induced ATP depletion represses mTOR signaling in the absence of 
      increases in AMP-activated protein kinase activity in the rat liver.
PG  - 1984-8
AB  - Administration of ethionine to female rats caused a rapid and severe decline in 
      liver ATP and inhibited hepatic protein synthesis in association with 
      hypophosphorylation of eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) 
      and 70-kDa ribosomal protein S6 kinase (S6K1), two key regulatory proteins 
      involved in initiation of mRNA translation. Phosphorylation of both regulatory 
      proteins is mediated through a signaling pathway that involves the mammalian 
      target of rapamycin (mTOR). Recent studies indicate that AMP-activated protein 
      kinase (AMPK) plays a role in the cellular response to environmental stresses, 
      which deplete ATP, and suppresses protein synthesis through downregulated mTOR 
      signaling. We investigated the possible involvement of AMPK in the 
      ethionine-induced inhibition of protein synthesis. The administration of 
      ethionine surprisingly decreased AMPK activity compared with controls despite ATP 
      depletion. We conclude that inhibition of protein synthesis by ethionine is due 
      to AMPK-independent inhibition of mTOR signaling following ATP depletion.
FAU - Yoshizawa, Fumiaki
AU  - Yoshizawa F
AD  - Department of Animal Science, Utsunomiya University, Utsunomiya, Tochigi 
      321-8205, Japan. fumiaki@cc.utsunomiya-u.ac.jp
FAU - Mochizuki, Shinji
AU  - Mochizuki S
FAU - Doi, Masako
AU  - Doi M
FAU - Yamaoka, Ippei
AU  - Yamaoka I
FAU - Sugahara, Kunio
AU  - Sugahara K
LA  - eng
PT  - Journal Article
DEP - 20090907
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - WX1BN24WZT (Ethionine)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Ethionine/*pharmacology
MH  - Female
MH  - Liver/*drug effects/enzymology
MH  - Phosphorylation
MH  - Protein Kinases/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*drug effects
MH  - TOR Serine-Threonine Kinases
EDAT- 2009/09/08 06:00
MHDA- 2010/01/01 06:00
CRDT- 2009/09/08 06:00
PHST- 2009/09/08 06:00 [entrez]
PHST- 2009/09/08 06:00 [pubmed]
PHST- 2010/01/01 06:00 [medline]
AID - JST.JSTAGE/bbb/90154 [pii]
AID - 10.1271/bbb.90154 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2009 Sep;73(9):1984-8. doi: 10.1271/bbb.90154. Epub 
      2009 Sep 7.