PMID- 19734969
OWN - NLM
STAT- MEDLINE
DCOM- 20091112
LR  - 20190816
IS  - 1651-2057 (Electronic)
IS  - 0001-5555 (Linking)
VI  - 89
IP  - 5
DP  - 2009
TI  - Sterile trauma to normal human dermis invariably induces IL1beta, IL6 and IL8 in 
      an innate response to "danger".
PG  - 459-65
LID - 10.2340/00015555-0683 [doi]
AB  - Microdialysis allows the study of the local production and temporal resolution of 
      cytokines in living skin. Samples were taken from the normal skin of 10 healthy 
      subjects for 24-28 h after insertion of a concentric microdialysis catheter, and 
      analysed with a Luminex bead-based assay. Interleukin-1 beta (IL1b), IL6 and IL8 
      were seen in all subjects at all time-points after the first hour. Levels peaked 
      at 5-8 h, equilibrating to lower levels at 24 h. Immunohistological double 
      staining for human leukocyte antigen (HLA)-DR and intracellular cytokines on 
      biopsies taken after catheter removal showed many stained cells in the dermis, in 
      contrast to the few cells stained in the epidermis. This study demonstrates the 
      reactive capability of the dermis when provoked separately from the epidermis. 
      The production of IL1b, IL6 and IL8 occurs invariably in what can be termed an 
      innate, dermal response to "danger"; in this case in the form of sterile needle 
      trauma.
FAU - Sjogren, Florence
AU  - Sjogren F
AD  - Department of Clinicial and Experimental Medicine, Division of Dermatology, 
      University Hospital, Linkoping, Sweden.
FAU - Anderson, Chris
AU  - Anderson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Acta Derm Venereol
JT  - Acta dermato-venereologica
JID - 0370310
RN  - 0 (CXCL8 protein, human)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Interleukins)
SB  - IM
MH  - Adult
MH  - Asepsis
MH  - Biopsy
MH  - Catheterization/*adverse effects
MH  - Dermis/*immunology/injuries
MH  - Epidermis/immunology/injuries
MH  - Female
MH  - Forearm
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - *Immunity, Innate
MH  - Immunohistochemistry
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Interleukins/*metabolism
MH  - Male
MH  - Microdialysis/*adverse effects
MH  - Middle Aged
MH  - Punctures/adverse effects
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2009/09/08 06:00
MHDA- 2009/11/13 06:00
CRDT- 2009/09/08 06:00
PHST- 2009/09/08 06:00 [entrez]
PHST- 2009/09/08 06:00 [pubmed]
PHST- 2009/11/13 06:00 [medline]
AID - 10.2340/00015555-0683 [doi]
PST - ppublish
SO  - Acta Derm Venereol. 2009;89(5):459-65. doi: 10.2340/00015555-0683.