PMID- 19736311
OWN - NLM
STAT- MEDLINE
DCOM- 20091207
LR  - 20220711
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 45
DP  - 2009 Nov 6
TI  - Src-dependent STAT-3-mediated expression of monocyte chemoattractant protein-1 is 
      required for 15(S)-hydroxyeicosatetraenoic acid-induced vascular smooth muscle 
      cell migration.
PG  - 31142-55
LID - 10.1074/jbc.M109.012526 [doi]
AB  - To understand the role of human 15-lipoxygenase 1 (15-LOX1) in vascular wall 
      remodeling, we have studied the effect of the major 15-LOX1 metabolite of 
      arachidonic acid, 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE), on vascular 
      smooth muscle cell (VSMC) migration both in vitro and in vivo. Among 5(S)-HETE, 
      12(S)-HETE, and 15(S)-HETE, 15(S)-HETE potentially stimulated more vascular 
      smooth muscle cell (VSMC) migration. In addition, 15(S)-HETE-induced VSMC 
      migration was dependent on Src-mediated activation of signal transducer and 
      activator of transcription-3 (STAT-3). 15(S)-HETE also induced monocyte 
      chemoattractant protein-1 (MCP-1) expression via Src-STAT-3 signaling, and 
      neutralizing anti-MCP-1 antibodies completely negated 15(S)-HETE-induced VSMC 
      migration. Cloning and characterization of a 2.6-kb MCP-1 promoter revealed the 
      presence of four putative STAT-binding sites, and the site that is proximal to 
      the transcription start site was found to be essential for 15(S)-HETE-induced 
      Src-STAT-3-mediated MCP-1 expression. Rat carotid arteries that were subjected to 
      balloon injury and transduced with Ad-15-LOX1 upon exposure to [(3)H]arachidonic 
      acid ex vivo produced 15-HETE as a major eicosanoid and enhanced balloon 
      injury-induced expression of MCP-1 in smooth muscle cells in Src and 
      STAT-3-dependent manner in vivo. Adenovirus-mediated delivery of 15-LOX1 into rat 
      carotid artery also led to recruitment and homing of macrophages to medial region 
      in response to injury. In addition, transduction of Ad-15-LOX1 into arteries 
      enhanced balloon injury-induced smooth muscle cell migration from media to intima 
      and neointima formation. These results show for the first time that 
      15-LOX1-15(S)-HETE axis plays a major role in vascular wall remodeling after 
      balloon angioplasty.
FAU - Potula, Harihara S K
AU  - Potula HS
AD  - Department of Physiology, The University of Tennessee Health Science Center, 
      Memphis, Tennessee 38163, USA.
FAU - Wang, Dong
AU  - Wang D
FAU - Quyen, Dong Van
AU  - Quyen DV
FAU - Singh, Nikhlesh K
AU  - Singh NK
FAU - Kundumani-Sridharan, Venkatesh
AU  - Kundumani-Sridharan V
FAU - Karpurapu, Manjula
AU  - Karpurapu M
FAU - Park, Edwards A
AU  - Park EA
FAU - Glasgow, Wayne C
AU  - Glasgow WC
FAU - Rao, Gadiparthi N
AU  - Rao GN
LA  - eng
GR  - R01 HL064165/HL/NHLBI NIH HHS/United States
GR  - HL064165/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090907
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
SB  - IM
MH  - Animals
MH  - Arachidonate 15-Lipoxygenase/genetics/metabolism
MH  - Cell Line
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/*metabolism
MH  - In Vitro Techniques
MH  - Myocytes, Smooth Muscle/*cytology/metabolism
MH  - Proto-Oncogene Proteins pp60(c-src)/genetics/*metabolism
MH  - Rats
MH  - STAT3 Transcription Factor/genetics/*metabolism
MH  - Signal Transduction
PMC - PMC2781513
EDAT- 2009/09/09 06:00
MHDA- 2009/12/16 06:00
PMCR- 2010/11/06
CRDT- 2009/09/09 06:00
PHST- 2009/09/09 06:00 [entrez]
PHST- 2009/09/09 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2010/11/06 00:00 [pmc-release]
AID - S0021-9258(20)38016-9 [pii]
AID - M109.012526 [pii]
AID - 10.1074/jbc.M109.012526 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 Nov 6;284(45):31142-55. doi: 10.1074/jbc.M109.012526. Epub 2009 
      Sep 7.