PMID- 19736868
OWN - NLM
STAT- MEDLINE
DCOM- 20091009
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 31
IP  - 4
DP  - 2009 Aug
TI  - Comparison of fluorescence in situ hybridization, hybrid capture 2 and polymerase 
      chain reaction for the detection of high-risk human papillomavirus in cervical 
      cytology specimens.
PG  - 208-16
AB  - OBJECTIVE: To compare a recently developed fluorescence in situ hybridization 
      (FISH) high-risk human papillomavirus (HR-HPV) assay to Hybrid Capture 2 (HC2) 
      (Digene Corporation, Gaithersburg, Maryland, U.S.A.) and polymerase chain 
      reaction (PCR) for the detection of HR-HPV subtypes in cervical cytology 
      specimens. STUDY DESIGN: One hundred forty-one liquid-based cytology specimens 
      were used to produce a thin-layer slide for FISH analysis. The remaining material 
      was sent for HC2 and PCR HR-HPV testing. Thin-layer slides were hybridized with a 
      FISH probe set containing a biotin-labeled HR-HPV cocktail and were manually 
      screened for HR-HPV-infected cells. Specimens with > or = 1 HPV-positive cell by 
      FISH were considered positive for HR-HPV infection. RESULTS: There was complete 
      concordance between HC2, FISH and PCR in 104 (75%) specimens. FISH was concordant 
      with HC2 and PCR in 120 (85%) and 115 (82%) specimens, respectively. HC2 and PCR 
      were concordant in 118 (84%) specimens. CONCLUSION: The concordance of HR-HPV 
      detection between FISH and HC2/PCR appears similar to concordances between HC2 
      and PCR. This suggests that FISH may be another method of detecting HR-HPV while 
      having the potential to add additional information such as integrated/episomal 
      staining and the ability to detect chromosomal abnormalities in individual cells.
FAU - Voss, Jesse S
AU  - Voss JS
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 
      Rochester, Minnesota 55905, USA.
FAU - Kipp, Benjamin R
AU  - Kipp BR
FAU - Campion, Michael B
AU  - Campion MB
FAU - Sokolova, Irina A
AU  - Sokolova IA
FAU - Henry, Michael R
AU  - Henry MR
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Clayton, Amy C
AU  - Clayton AC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (DNA Probes, HPV)
SB  - IM
MH  - Cervix Uteri/pathology/*virology
MH  - DNA Probes, HPV
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Diagnostic Techniques/*methods
MH  - Nucleic Acid Hybridization/*methods
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Papillomavirus Infections/diagnosis
MH  - Polymerase Chain Reaction/*methods
MH  - Vaginal Smears
EDAT- 2009/09/10 06:00
MHDA- 2009/10/10 06:00
CRDT- 2009/09/10 06:00
PHST- 2009/09/10 06:00 [entrez]
PHST- 2009/09/10 06:00 [pubmed]
PHST- 2009/10/10 06:00 [medline]
PST - ppublish
SO  - Anal Quant Cytol Histol. 2009 Aug;31(4):208-16.