PMID- 1973747
OWN - NLM
STAT- MEDLINE
DCOM- 19900830
LR  - 20190510
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 14
IP  - 3
DP  - 1990 May-Jun
TI  - Application of the Syva EMIT and Abbott TDx amphetamine immunoassays to the 
      detection of 3,4-methylene-dioxymethamphetamine (MDMA) and 
      3,4-methylene-dioxyethamphetamine (MDEA) in urine.
PG  - 149-53
AB  - MDMA and MDEA are hallucinogenic analogs of amphetamine. The need for laboratory 
      monitoring of these substances has developed as a result of their increased 
      recreational use. Since the Abbott TDx and Syva EMIT-d.a.u. immunoassays are 
      commonly used tests for urine monitoring of drugs-of-abuse, the amphetamine assay 
      of each manufacturer was assessed to determine the degree of cross-reactivity 
      with MDMA and MDEA. Cross-reactivity was evaluated using a series of MDMA- and 
      MDEA-spiked urine samples. Testing was performed over a two-day period with 3 
      runs/day and each sample run in duplicate. The Syva EMIT d.a.u. amphetamine assay 
      was positive only at the highest concentration standard (10.0 micrograms/mL) for 
      both MDMA and MDEA. Consequently, no further testing was performed with this 
      assay. Calibration curves were generated for the TDx runs and percent 
      cross-reactivity determinations were made. Precision for the TDx data was 
      evaluated based on within-day and between-day coefficients of variation (CV). CVs 
      for MDMA runs were below 6% for within-day and below 5% for between-day runs. 
      Values of CV for MDEA were below 16% for both within-day and between runs; CVs 
      were less than 2.5% for positive values. Cross-reactivity for MDMA ranged from 
      18% (10.00 micrograms/mL) to 118% (0.15 microgram/mL). Cross-reactivity for the 
      MDEA standards ranged from 12% (10.00 micrograms/mL) to 47% (0.15 micrograms/mL). 
      The presence of MDMA and/or MDEA in samples resulting in a negative EMIT-d.a.u. 
      test and a positive TDx test was confirmed by GC/MS analysis.(ABSTRACT TRUNCATED 
      AT 250 WORDS)
FAU - Kunsman, G W
AU  - Kunsman GW
AD  - Department of Pharmacology, Louisiana State University Medical Center, Shreveport 
      71130.
FAU - Manno, J E
AU  - Manno JE
FAU - Cockerham, K R
AU  - Cockerham KR
FAU - Manno, B R
AU  - Manno BR
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - ML1I4KK67B (3,4-methylenedioxyethamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/*urine
MH  - Amphetamines/*urine
MH  - Chemistry Techniques, Analytical
MH  - Cross Reactions
MH  - Dose-Response Relationship, Drug
MH  - False Negative Reactions
MH  - Fluorescence Polarization/methods
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Reproducibility of Results
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
AID - 10.1093/jat/14.3.149 [doi]
PST - ppublish
SO  - J Anal Toxicol. 1990 May-Jun;14(3):149-53. doi: 10.1093/jat/14.3.149.