PMID- 19737531
OWN - NLM
STAT- MEDLINE
DCOM- 20091130
LR  - 20091026
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 390
IP  - 2
DP  - 2009 Dec 11
TI  - Early growth response-1 is involved in foam cell formation and is upregulated by 
      the TLR9-MyD88-ERK1/2 pathway.
PG  - 196-200
LID - 10.1016/j.bbrc.2009.09.009 [doi]
AB  - Early growth response-1 (Egr-1), a zinc finger transcription factor, plays a key 
      regulatory role in pathological cardiovascular processes including 
      atherosclerosis. Here, we investigate whether Egr-1 expression and foam cell 
      formation require toll-like receptor 9 (TLR9) and myeloid differentiation factor 
      88 (MyD88). CpG DNA and its related synthetic CpG oligodeoxynucleotides (CpG ODN) 
      play an important role in immunity and inflammation. CpG ODN increased expression 
      of Egr-1 and formation of foam cells in Raw264.7 cells or bone marrow-derived 
      macrophages. Egr-1 siRNA decreased foam cell formation by CpG ODN compared to 
      that of control siRNA. In addition, when TLR9 or MyD88 was knocked down, CpG 
      ODN-induced Egr-1 expression was also attenuated. CpG ODN increased ERK1/2 
      phosphorylation. U0126, a MEK pathway inhibitor, suppressed activation of Egr-1 
      expression by CpG ODN. CpG ODN-induced expression of tissue factor (TF) and 
      NGFI-A binding protein 2 (Nab2), and the expression of both genes is blocked by 
      knockdown of TLR9 or MyD88 siRNA or MEK inhibition. These results suggest that 
      CpG ODN activates the TLR9-MyD88-ERK1/2 pathway causing expression of Egr-1 and 
      its target genes such as TF and Nab2, thus inducing foam cell formation.
FAU - Kim, Jin-Sik
AU  - Kim JS
AD  - Aging-Associated Vascular Disease Research Center, Department of Biochemistry & 
      Molecular Biology, College of Medicine, Yeungnam University, Daegu 705-717, South 
      Korea.
FAU - Park, Dae-Weon
AU  - Park DW
FAU - Lee, Hyung-Kyoung
AU  - Lee HK
FAU - Kim, Jae-Ryong
AU  - Kim JR
FAU - Baek, Suk-Hwan
AU  - Baek SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090906
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Nab2 protein, mouse)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Repressor Proteins)
RN  - 0 (Toll-Like Receptor 9)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Early Growth Response Protein 1/genetics/*physiology
MH  - Foam Cells/metabolism/*physiology
MH  - Gene Expression Regulation
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Neoplasm Proteins/genetics
MH  - Oligodeoxyribonucleotides/genetics
MH  - Repressor Proteins/genetics
MH  - Thromboplastin/genetics
MH  - Toll-Like Receptor 9/metabolism
MH  - Up-Regulation
EDAT- 2009/09/10 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/10 06:00
PHST- 2009/08/28 00:00 [received]
PHST- 2009/09/02 00:00 [accepted]
PHST- 2009/09/10 06:00 [entrez]
PHST- 2009/09/10 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0006-291X(09)01786-0 [pii]
AID - 10.1016/j.bbrc.2009.09.009 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Dec 11;390(2):196-200. doi: 
      10.1016/j.bbrc.2009.09.009. Epub 2009 Sep 6.