PMID- 19737562
OWN - NLM
STAT- MEDLINE
DCOM- 20091113
LR  - 20131121
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 583
IP  - 19
DP  - 2009 Oct 6
TI  - Functional assignment of Glu386 and Arg388 in the active site of 
      L-galactono-gamma-lactone dehydrogenase.
PG  - 3199-203
LID - 10.1016/j.febslet.2009.09.004 [doi]
AB  - The flavoenzyme L-galactono-gamma-lactone dehydrogenase (GALDH) catalyzes the 
      terminal step of vitamin C biosynthesis in plants. Little is known about the 
      catalytic mechanism of GALDH and related aldonolactone oxidoreductases. Here we 
      identified an essential Glu-Arg pair in the active site of GALDH from Arabidopsis 
      thaliana. Glu386 and Arg388 variants show high K(m) values for 
      L-galactono-1,4-lactone and low turnover rates. Arg388 is crucial for the 
      stabilization of the anionic form of the reduced FAD cofactor. Glu386 is involved 
      in productive substrate binding. The E386D variant has lost its specificity for 
      L-galactono-1,4-lactone and shows the highest catalytic efficiency with 
      L-gulono-1,4-lactone.
FAU - Leferink, Nicole G H
AU  - Leferink NG
AD  - Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA 
      Wageningen, The Netherlands. m.f.jose@rug.nl
FAU - Jose, Mac Donald F
AU  - Jose MD
FAU - van den Berg, Willy A M
AU  - van den Berg WA
FAU - van Berkel, Willem J H
AU  - van Berkel WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090906
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Lactones)
RN  - 0 (Sugar Acids)
RN  - 0 (galactono-1,4-lactone)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.2.3 (galactonolactone dehydrogenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Arabidopsis/genetics/*metabolism
MH  - Arginine/genetics/*metabolism
MH  - Catalytic Domain/genetics
MH  - Conserved Sequence
MH  - Glutamic Acid/genetics/*metabolism
MH  - Lactones/*metabolism
MH  - Molecular Sequence Data
MH  - Oxidoreductases Acting on CH-CH Group Donors/genetics/*metabolism
MH  - Sugar Acids/*metabolism
EDAT- 2009/09/10 06:00
MHDA- 2009/11/17 06:00
CRDT- 2009/09/10 06:00
PHST- 2009/07/28 00:00 [received]
PHST- 2009/09/02 00:00 [revised]
PHST- 2009/09/03 00:00 [accepted]
PHST- 2009/09/10 06:00 [entrez]
PHST- 2009/09/10 06:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - S0014-5793(09)00688-7 [pii]
AID - 10.1016/j.febslet.2009.09.004 [doi]
PST - ppublish
SO  - FEBS Lett. 2009 Oct 6;583(19):3199-203. doi: 10.1016/j.febslet.2009.09.004. Epub 
      2009 Sep 6.