PMID- 19737990
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20091109
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Linking)
VI  - 39
IP  - 3
DP  - 2009 Nov 6
TI  - Changes in behavior and gene expression induced by caloric restriction in C57BL/6 
      mice.
PG  - 227-35
LID - 10.1152/physiolgenomics.00082.2009 [doi]
AB  - Caloric restriction (CR) is an effective method for prevention of age-associated 
      diseases as well as overweight and obesity; however, there is controversy 
      regarding the effects of dieting regimens on behavior. In this study, we 
      investigated two different dieting regimens: repeated fasting and refeeding (RFR) 
      and daily feeding of half the amount of food consumed by RFR mice (CR). CR and 
      RFR mice had an approximate 20% reduction in food intake compared with control 
      mice. Open field, light-dark transition, elevated plus maze, and forced swimming 
      tests indicated that CR, but not RFR, reduced anxiety- and depressive-like 
      behaviors, with a reduction peak on day 8. Using a mouse whole genome microarray, 
      we analyzed gene expression in the prefrontal cortex, amygdala, and hypothalamus. 
      In addition to the CR-responsive genes commonly modified by RFR and CR, each 
      regimen differentially changed the expression of distinct genes in each region. 
      The most profound change was observed in the amygdalas of CR mice: 884 genes were 
      specifically upregulated. Ingenuity pathway analysis revealed that these 884 
      genes significantly modified nine canonical pathways in the amygdala. 
      alpha-Adrenergic and dopamine receptor signalings were the two top-scoring 
      pathways. Quantitative RT-PCR confirmed the upregulation of six genes in these 
      pathways. Western blotting confirmed that CR specifically increased dopamine- and 
      cAMP-regulated phosphoprotein (Darpp-32), a key regulator of dopamine receptor 
      signaling, in the amygdala. Our results suggest that CR may change behavior 
      through altered gene expression.
FAU - Yamamoto, Yuta
AU  - Yamamoto Y
AD  - Department of Stress Science, Institute of Health Biosciences, The University of 
      Tokushima Graduate School, Tokushima 770-8503, Japan.
FAU - Tanahashi, Toshihito
AU  - Tanahashi T
FAU - Kawai, Tomoko
AU  - Kawai T
FAU - Chikahisa, Sachiko
AU  - Chikahisa S
FAU - Katsuura, Sakurako
AU  - Katsuura S
FAU - Nishida, Kensei
AU  - Nishida K
FAU - Teshima-Kondo, Shigetada
AU  - Teshima-Kondo S
FAU - Sei, Hiroyoshi
AU  - Sei H
FAU - Rokutan, Kazuhito
AU  - Rokutan K
LA  - eng
PT  - Journal Article
DEP - 20090908
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 0 (Dopamine and cAMP-Regulated Phosphoprotein 32)
RN  - 0 (Ppp1r1b protein, mouse)
SB  - IM
MH  - Amygdala/metabolism
MH  - Animals
MH  - Behavior, Animal/*physiology
MH  - Blotting, Western
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Dopamine and cAMP-Regulated Phosphoprotein 32/genetics/metabolism
MH  - Eating
MH  - *Gene Expression Profiling
MH  - Male
MH  - Maze Learning
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Motor Activity
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics/*physiology
MH  - Swimming
EDAT- 2009/09/10 06:00
MHDA- 2010/03/03 06:00
CRDT- 2009/09/10 06:00
PHST- 2009/09/10 06:00 [entrez]
PHST- 2009/09/10 06:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - 00082.2009 [pii]
AID - 10.1152/physiolgenomics.00082.2009 [doi]
PST - ppublish
SO  - Physiol Genomics. 2009 Nov 6;39(3):227-35. doi: 
      10.1152/physiolgenomics.00082.2009. Epub 2009 Sep 8.