PMID- 19740313
OWN - NLM
STAT- MEDLINE
DCOM- 20100125
LR  - 20221207
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 128
IP  - 1 Suppl
DP  - 2009 Sep
TI  - Selected commensal-related bacteria and Toll-like receptor 3 agonist 
      combinatorial codes synergistically induce interleukin-12 production by dendritic 
      cells to trigger a T helper type 1 polarizing programme.
PG  - e523-31
LID - 10.1111/j.1365-2567.2008.03022.x [doi]
AB  - Enteric infections remain a major health problem causing millions of deaths in 
      developing countries. The interplay among the host intestinal epithelium, the 
      mucosa-associated immune system and microbiota performs an essential role in gut 
      homeostasis and protection against infectious diseases. Dendritic cells (DCs) 
      play a key role in orchestrating protective immunity and tolerance in the gut. 
      The mechanisms by which DCs adapt their responses and discriminate between 
      virulent microbes and trillions of innocuous bacteria remain ill-defined. Here we 
      investigated the effect of cross-talk between commensal-related bacteria (CB) and 
      Toll-like receptor (TLR) agonists on DC activation and the outcome of the in 
      vitro T helper response. Human monocyte-derived DCs were exposed to eight 
      different Gram-positive or Gram-negative CB strains prior to activation with five 
      different TLR agonists. The key polarizing cytokines interleukin (IL)-12p70, 
      IL-10, IL-1beta and IL-6 were quantified and the fate of naive T-cell 
      differentiation was evaluated. We identified a unique combination of 
      Lactobacillus casei and TLR3 signals that acted in synergy to selectively 
      increase IL-12p70 secretion. Exposure to poly(I:C) converted L. casei-treated DCs 
      into potent promoters of T helper type 1 (Th1) responses. We propose that DCs can 
      integrate harmless and dangerous non-self signals delivered by viral products, to 
      mount robust Th1 responses. Thus, in vivo DC targeting with selective probiotics 
      may improve strategies for the management of enteric diseases.
FAU - Baba, Nobuyasu
AU  - Baba N
AD  - Immunoregulation Laboratory, CRCHUM, University of Montreal, Montreal, QC, 
      Canada.
FAU - Samson, Sandrine
AU  - Samson S
FAU - Bourdet-Sicard, Raphaelle
AU  - Bourdet-Sicard R
FAU - Rubio, Manuel
AU  - Rubio M
FAU - Sarfati, Marika
AU  - Sarfati M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081124
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Interferon Inducers)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptidoglycan)
RN  - 0 (TLR3 protein, human)
RN  - 0 (Toll-Like Receptor 3)
RN  - 12777-81-0 (Flagellin)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Dendritic Cells/drug effects/*immunology/microbiology
MH  - Flagellin/immunology
MH  - Humans
MH  - Interferon Inducers/pharmacology
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-12/agonists/*biosynthesis/immunology
MH  - Interleukin-1beta/biosynthesis/immunology
MH  - Interleukin-6/biosynthesis
MH  - Intestines/*immunology/microbiology
MH  - Lacticaseibacillus casei/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Peptidoglycan/immunology
MH  - Poly I-C/pharmacology
MH  - *Probiotics
MH  - Signal Transduction/drug effects/immunology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Th1 Cells/drug effects/*immunology/microbiology
MH  - Toll-Like Receptor 3/*agonists/metabolism
PMC - PMC2753912
EDAT- 2009/09/25 06:00
MHDA- 2010/01/26 06:00
PMCR- 2010/09/01
CRDT- 2009/09/11 06:00
PHST- 2009/09/11 06:00 [entrez]
PHST- 2009/09/25 06:00 [pubmed]
PHST- 2010/01/26 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - IMM3022 [pii]
AID - 10.1111/j.1365-2567.2008.03022.x [doi]
PST - ppublish
SO  - Immunology. 2009 Sep;128(1 Suppl):e523-31. doi: 10.1111/j.1365-2567.2008.03022.x. 
      Epub 2008 Nov 24.