PMID- 19745197
OWN - NLM
STAT- MEDLINE
DCOM- 20091203
LR  - 20141120
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 29
IP  - 12
DP  - 2009 Dec
TI  - Tumor necrosis factor-alpha potentiates RhoA-mediated monocyte transmigratory 
      activity in vivo at a picomolar level.
PG  - 2138-45
LID - 10.1161/ATVBAHA.109.195735 [doi]
AB  - OBJECTIVE: The serum level of tumor necrosis factor-alpha (TNF-alpha) is in the 
      picomolar range under inflammatory conditions. We investigated whether these 
      picomolar levels of TNF-alpha directly modulate the functional activities of 
      circulating monocytes. METHODS AND RESULTS: In THP-1 monocytes treated with 
      TNF-alpha (1 to 100 pmol/L/30 minutes), cytosolic RhoA small GTPase rapidly 
      translocated to the plasma membrane via functionally active ezrin/radixin/moesin 
      (ERM) complex, a cytoskeletal linker, and subsequent actin polymerization through 
      NF-kappaB activation. The threonine phosphorylation of ERM was accomplished by 
      the activation of TNF receptor type I (TNFRI) and signaling pathways involving 
      PI3K and an atypical PKC; ie, PKCzeta. The TNF-alpha-treated monocytes (10 
      pmol/L) displayed more potent and prolonged generation of GTP-bound RhoA in 
      response to secondary stimulation with RhoA-activating monocyte chemoattractant 
      protein-1 (MCP-1). Clearly, human circulating monocytes preconditioned by 10 
      pmol/L TNF-alpha augmented MCP-1-mediated chemotaxis and firm adhesion on VCAM-1 
      and ICAM-1 in vitro and ex vivo. The elevation of serum TNF-alpha (>5 pmol/L 
      within 16 hours), which was introduced by intraperitoneal injection of 
      mouse-specific TNF-alpha to C57/BL6 mice, enhanced the number of CD80+ monocytes 
      transmigrating to the JE/MCP-1-injected intraperitoneal space. CONCLUSIONS: 
      Picomolar concentrations of TNF-alpha in the bloodstream may prime the 
      RhoA-dependent activities of circulating monocytes to enhance recruitment to 
      active inflammatory foci.
FAU - Lim, Sunny
AU  - Lim S
AD  - Department of Internal Medicine, Asan Medical Center, University of Ulsan College 
      of Medicine, Seoul, South Korea.
FAU - Ryu, Jewon
AU  - Ryu J
FAU - Shin, Jin-Ae
AU  - Shin JA
FAU - Shin, Min-Jung
AU  - Shin MJ
FAU - Ahn, Yeong Ki
AU  - Ahn YK
FAU - Kim, Jae Joong
AU  - Kim JJ
FAU - Han, Ki Hoon
AU  - Han KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090910
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (ezrin)
RN  - 124671-05-2 (RHOA protein, human)
RN  - 144131-77-1 (moesin)
RN  - 144517-21-5 (radixin)
RN  - EC 3.6.5.2 (RhoA protein, mouse)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active/drug effects
MH  - Cell Line
MH  - Cell Membrane/drug effects/metabolism
MH  - Cell Movement/drug effects/physiology
MH  - Chemokine CCL2/pharmacology
MH  - Cytoskeletal Proteins/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Macrophages, Peritoneal/drug effects/physiology
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microfilament Proteins/metabolism
MH  - Monocytes/*drug effects/*physiology
MH  - NF-kappa B/metabolism
MH  - Peritoneal Cavity/cytology
MH  - Phosphorylation
MH  - Receptors, Tumor Necrosis Factor, Type I/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/administration & dosage/*pharmacology
MH  - rho GTP-Binding Proteins/*physiology
MH  - rhoA GTP-Binding Protein/*physiology
EDAT- 2009/09/12 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/12 06:00
PHST- 2009/09/12 06:00 [entrez]
PHST- 2009/09/12 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - ATVBAHA.109.195735 [pii]
AID - 10.1161/ATVBAHA.109.195735 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2009 Dec;29(12):2138-45. doi: 
      10.1161/ATVBAHA.109.195735. Epub 2009 Sep 10.