PMID- 19745547 OWN - NLM STAT- MEDLINE DCOM- 20091231 LR - 20190827 IS - 1348-2246 (Electronic) IS - 0910-6340 (Linking) VI - 25 IP - 9 DP - 2009 Sep TI - Immobilization of glucose oxidase on a poly(dimethylsiloxane) layer by using poly(L-lysine) as a polymer backbone. PG - 1159-62 AB - Poly(dimethylsiloxane) (PDMS)-coated platinum electrodes modified with glucose oxidase (GOx) have been prepared from poly(L-lysine) (polymer backbone), glutaraldehyde (cross-linking agent) and poly(ethylene glycol) units. To fabricate a GOx layer by applying cross-linking chemistries, the PDMS layer was treated with oxygen plasma to replace silane groups with silanol groups. Optimization for the chemical fabrication of a GOx layer resulted in a simple preparation of sensors with a wide detectable range (0.1-6.0 mM) and without interference from hydrogen peroxide produced by a GOx reaction and the other in biological samples. FAU - Yasukawa, Tomoyuki AU - Yasukawa T AD - Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan. yasu@sci.u-hyogo.ac.jp FAU - Maekawa, Eiji AU - Maekawa E FAU - Mizutani, Fumio AU - Mizutani F LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Anal Sci JT - Analytical sciences : the international journal of the Japan Society for Analytical Chemistry JID - 8511078 RN - 0 (Blood Glucose) RN - 0 (Dimethylpolysiloxanes) RN - 0 (Enzymes, Immobilized) RN - 0F5N573A2Y (Ether) RN - 25104-18-1 (Polylysine) RN - 3WJQ0SDW1A (Polyethylene Glycols) RN - 49DFR088MY (Platinum) RN - EC 1.1.3.4 (Glucose Oxidase) RN - IY9XDZ35W2 (Glucose) RN - T3C89M417N (Glutaral) SB - IM MH - Adsorption MH - Blood Glucose/analysis MH - Dimethylpolysiloxanes/*chemistry MH - Electrodes MH - Enzymes, Immobilized/*chemistry MH - Ether/chemistry MH - Glucose/analysis MH - Glucose Oxidase/*chemistry MH - Glutaral/chemistry MH - Platinum/chemistry MH - Polyethylene Glycols/chemistry MH - Polylysine/*chemistry EDAT- 2009/09/12 06:00 MHDA- 2010/01/01 06:00 CRDT- 2009/09/12 06:00 PHST- 2009/09/12 06:00 [entrez] PHST- 2009/09/12 06:00 [pubmed] PHST- 2010/01/01 06:00 [medline] AID - JST.JSTAGE/analsci/25.1159 [pii] AID - 10.2116/analsci.25.1159 [doi] PST - ppublish SO - Anal Sci. 2009 Sep;25(9):1159-62. doi: 10.2116/analsci.25.1159.