PMID- 19746735
OWN - NLM
STAT- MEDLINE
DCOM- 20091016
LR  - 20190715
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 43
IP  - 16
DP  - 2009 Aug 15
TI  - Hepatic transcriptomic and metabolomic responses in the stickleback (Gasterosteus 
      aculeatus) exposed to environmentally relevant concentrations of 
      dibenzanthracene.
PG  - 6341-8
AB  - A three-spined stickleback (Gasterosteus aculeatus) cDNA array and 
      one-dimensional 1H nuclear magnetic resonance (NMR) spectroscopy-based 
      metabolomics approach, together with individual biomarkers,were employed to 
      investigate the responses of male sticklebacks to polycyclic aromatic hydrocarbon 
      exposure. Fish were exposed to 1,2:5,6-dibenzanthracene (DbA) at concentrations 
      between 0.01 and 50 microg per liter dissolved in the ambient water for four 
      days, and hepatic transcript and metabolite profiles were determined in 
      comparison with those of solvent-exposed controls. Induction of gene expression 
      was apparent for cytochrome P450 1A (CYP1A) and CYP2-family monooxygenases and 
      these responses were strongly correlated with DbA exposure concentrations (for 
      CYP1A r > 0.996). Expression of suites of genes related to bile acid 
      biosynthesis, steroid metabolism, and endocrine function were also affected, as 
      demonstrated by gene ontology analyses. Expression changes in selected genes were 
      confirmed by real-time PCR. Metabolomics highlighted notable changes in 
      concentrations of taurine, malonate, glutamate, and alanine. These statistically 
      significant responses to environmentally relevant concentrations of DbA at the 
      transcriptomic and metabolomic levels provided sensitive markers characteristic 
      of environmentally relevant low-level DbA exposure. Metabolic pathways were 
      identified where both gene expression and metabolite concentrations were altered 
      in response to DbA.
FAU - Williams, Tim D
AU  - Williams TD
AD  - School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK. 
      t.d.williams@bham.ac.uk
FAU - Wu, Huifeng
AU  - Wu H
FAU - Santos, Eduarda M
AU  - Santos EM
FAU - Ball, Jon
AU  - Ball J
FAU - Katsiadaki, Ioanna
AU  - Katsiadaki I
FAU - Brown, Margaret M
AU  - Brown MM
FAU - Baker, Paul
AU  - Baker P
FAU - Ortega, Fernando
AU  - Ortega F
FAU - Falciani, Francesco
AU  - Falciani F
FAU - Craft, John A
AU  - Craft JA
FAU - Tyler, Charles R
AU  - Tyler CR
FAU - Chipman, James K
AU  - Chipman JK
FAU - Viant, Mark R
AU  - Viant MR
LA  - eng
GR  - 6/JIF13209/Biotechnology and Biological Sciences Research Council/United Kingdom
GR  - G.4500017/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Benz(a)Anthracenes)
RN  - 0 (Steroids)
RN  - 0 (dibenzanthracenes)
SB  - IM
MH  - Animals
MH  - Benz(a)Anthracenes/administration & dosage/*toxicity
MH  - Endocrine System/drug effects/metabolism
MH  - Environmental Exposure/analysis
MH  - Environmental Monitoring
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Liver/*drug effects/*metabolism
MH  - Male
MH  - Metabolic Detoxication, Phase I
MH  - Metabolic Networks and Pathways/drug effects
MH  - *Metabolomics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Principal Component Analysis
MH  - Smegmamorpha/*genetics/*metabolism
MH  - Steroids/metabolism
MH  - Toxicity Tests
EDAT- 2009/09/15 06:00
MHDA- 2009/10/17 06:00
CRDT- 2009/09/15 06:00
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2009/10/17 06:00 [medline]
AID - 10.1021/es9008689 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2009 Aug 15;43(16):6341-8. doi: 10.1021/es9008689.