PMID- 19746742
OWN - NLM
STAT- MEDLINE
DCOM- 20091016
LR  - 20211203
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 43
IP  - 16
DP  - 2009 Aug 15
TI  - Transactivation potencies of Baikal seal constitutive active/androstane receptor 
      by persistent organic pollutants and brominated flame retardants.
PG  - 6391-7
AB  - To characterize ligand-dependent transcriptional activation of constitutive 
      active/androstane receptor (CAR) in aquatic mammals, transactivation potentials 
      of the Baikal seal (Pusa sibirica) CAR (bsCAR) by environmental pollutants, 
      including persistent organic pollutants (POPs) and brominated flame retardants 
      (BFRs), were investigated using an in vitro reporter gene assay, and compared 
      with those of the mouse CAR (mCAR). Measurement of luciferase reporter gene 
      activities demonstrated that the seal CAR was activated by POPs, including a 
      technical mixture of PCBs (Kanechlor-500), certain individual PCB congeners, DDT 
      compounds, and trans-nonachlor. No or slight bsCAR-dependent activity was 
      detected in experiments with PBDE congeners and HBCDs. The interspecies 
      comparison of lowest observed effect concentration (LOEC) for CAR transactivation 
      byeach compound revealed that bsCAR responds more sensitively to PCBs than mCAR. 
      In addition, bsCAR was weakly deactivated by PBDE99, whereas mCAR transcriptional 
      activity decreased weakly by PBDE100, PBDE154, and PBDE187. Comparison of 
      reporter gene activities by the congeners with the same IUPAC numbers among PCBs 
      and PBDEs revealed that both bsCAR and mCAR were not activated by PBDE99 and 
      PBDE153, but were activated by PCB99 and PCB153. The small ligand-binding pocket 
      in CAR may contribute to difference in response between PCBs and PBDEs. Given 
      that ethical rationale prevents dosing studies with such organohalogens in 
      aquatic mammals, our in vitro assay system constructed with CAR cDNA from a 
      species of interest provides a useful and realistic alternative approach in 
      ecotoxicology.
FAU - Sakai, Hiroki
AU  - Sakai H
AD  - Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, 
      Matsuyama 790-8577, Japan.
FAU - Kim, Eun-Young
AU  - Kim EY
FAU - Petrov, Evgeny A
AU  - Petrov EA
FAU - Tanabe, Shinsuke
AU  - Tanabe S
FAU - Iwata, Hisato
AU  - Iwata H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Constitutive Androstane Receptor)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Flame Retardants)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Organic Chemicals)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
SB  - IM
MH  - Animals
MH  - Caniformia/*genetics
MH  - Constitutive Androstane Receptor
MH  - Environmental Pollutants/*toxicity
MH  - Flame Retardants/*toxicity
MH  - Genes, Reporter
MH  - Hydrocarbons, Brominated/*toxicity
MH  - Mice
MH  - Organic Chemicals/*toxicity
MH  - Receptors, Cytoplasmic and Nuclear/*genetics/metabolism
MH  - Species Specificity
MH  - Transcriptional Activation/*drug effects
EDAT- 2009/09/15 06:00
MHDA- 2009/10/17 06:00
CRDT- 2009/09/15 06:00
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2009/10/17 06:00 [medline]
AID - 10.1021/es901120r [doi]
PST - ppublish
SO  - Environ Sci Technol. 2009 Aug 15;43(16):6391-7. doi: 10.1021/es901120r.