PMID- 19747464
OWN - NLM
STAT- MEDLINE
DCOM- 20100105
LR  - 20220129
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1301
DP  - 2009 Dec 8
TI  - Expression of kinase interacting with stathmin (KIS, UHMK1) in human brain and 
      lymphoblasts: Effects of schizophrenia and genotype.
PG  - 197-206
LID - 10.1016/j.brainres.2009.08.090 [doi]
AB  - Single nucleotide polymorphisms (SNPs) within the gene encoding the 
      serine/threonine kinase KIS (Kinase Interacting with Stathmin, also known as 
      UHMK1) have recently been associated with schizophrenia. As none of the disease 
      associated SNPs are coding, they may confer susceptibility by altering some facet 
      of KIS expression. Here we have characterised the cellular distribution of KIS in 
      human brain using in situ hybridisation and immunohistochemistry, and quantified 
      KIS protein and mRNA in two large brain series to determine if KIS expression is 
      altered in schizophrenia or bipolar disorder or in relation to a 
      schizophrenia-associated SNP (rs7513662). Post-mortem tissue from the superior 
      temporal gyrus of schizophrenia and control subjects, and also dorsolateral 
      prefrontal cortex, anterior cingulate cortex, and cerebellum from schizophrenia, 
      bipolar disorder, and control subjects were used. KIS expression was measured by 
      quantitative PCR (mRNA) and immunoautoradiography (protein), and was also 
      quantified by immunoblot in lymphoblast cell lines derived from schizophrenia and 
      control subjects. Our results demonstrate that KIS is expressed in neurons, and 
      its encoded protein is localised to the nucleus and cytoplasm. No difference in 
      KIS expression was found between diagnostic groups, or in the lymphoblast cell 
      lines, and no effect of rs7513662 genotype on KIS expression was found. Hence, 
      these data do not provide support for the hypothesis that altered expression is 
      the mechanism by which genetic variation of KIS may increase susceptibility to 
      schizophrenia, nor evidence that KIS expression is altered in the disease itself, 
      at least in terms of the parameters studied here.
FAU - Bristow, Greg C
AU  - Bristow GC
AD  - University Department of Psychiatry, Warneford Hospital, Oxford, UK.
FAU - Lane, Tracy A
AU  - Lane TA
FAU - Walker, Mary
AU  - Walker M
FAU - Chen, Li
AU  - Chen L
FAU - Sei, Yoshi
AU  - Sei Y
FAU - Hyde, Thomas M
AU  - Hyde TM
FAU - Kleinman, Joel E
AU  - Kleinman JE
FAU - Harrison, Paul J
AU  - Harrison PJ
FAU - Eastwood, Sharon L
AU  - Eastwood SL
LA  - eng
GR  - G0500180/MRC_/Medical Research Council/United Kingdom
GR  - Z01 MH002903-01/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090909
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (UHMK1 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Analysis of Variance
MH  - Autoradiography
MH  - Blotting, Western
MH  - Brain/*metabolism
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression/*genetics
MH  - *Genotype
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Intracellular Signaling Peptides and Proteins/*genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Neurons/metabolism
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Protein Serine-Threonine Kinases/*genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Schizophrenia/*genetics/metabolism
PMC - PMC2783906
MID - NIHMS144750
EDAT- 2009/09/15 06:00
MHDA- 2010/01/06 06:00
PMCR- 2010/12/08
CRDT- 2009/09/15 06:00
PHST- 2009/06/19 00:00 [received]
PHST- 2009/08/25 00:00 [revised]
PHST- 2009/08/26 00:00 [accepted]
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2010/01/06 06:00 [medline]
PHST- 2010/12/08 00:00 [pmc-release]
AID - S0006-8993(09)01867-8 [pii]
AID - 10.1016/j.brainres.2009.08.090 [doi]
PST - ppublish
SO  - Brain Res. 2009 Dec 8;1301:197-206. doi: 10.1016/j.brainres.2009.08.090. Epub 
      2009 Sep 9.