PMID- 19748164
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20220321
IS  - 1532-1983 (Electronic)
IS  - 0261-5614 (Linking)
VI  - 29
IP  - 3
DP  - 2010 Jun
TI  - Inhibition of JNK along with activation of ERK1/2 MAPK pathways improve 
      steatohepatitis among the rats.
PG  - 381-5
LID - 10.1016/j.clnu.2009.08.014 [doi]
AB  - BACKGROUND & AIMS: Oxidative stress-induced hepatocyte apoptosis is implicated in 
      the onset of non-alcoholic steatohepatitis (NASH). Regarding the pronounced roles 
      of mitogen activated protein kinases (MAPKs) in oxidative stress-induced cellular 
      damages and the development of NASH, we evaluated the effect of Teucrium polium 
      ethyl acetate (EtoAc) extract on rats with NASH and on the modulation of MAPKs 
      activities. METHODS: To induce NASH, a methionine choline deficient diet (MCD) 
      was given to N-Mary rats. These animals were then compared to the control group 
      receiving normal diet and/or MCD diet plus EtoAc extract (0.5g/kg/day). After 8 
      weeks, liver histopathology, malondialdehyde (MDA) and immunoblot analyses of 
      caspase-3 cleavage, ERK/pERK and JNK/pJNK were examined. RESULTS: Simultaneous 
      treatments with MCD diet and the EtoAc extract resulted in pronounced 
      improvements in liver steatosis, ballooning degeneration and inflammation. 
      Elevated MDA and caspase-3 levels among MCD-fed rats were also markedly 
      decreased. In addition, the EtoAc extract treatments caused significant reduction 
      in the phosphorylated form of JNK along with an increase in the phosphorylated 
      level of ERK1/2. CONCLUSION: These results indicate that the anti-apoptotic 
      effect of T. polium extract is mediated through inhibition of ROS generation. 
      Moreover, the inhibitory effect of T. polium on the development and progression 
      of NASH is apparently governed by the attenuation of JNK activation and 
      augmentation of ERK1/2 activity through phosphorylation.
CI  - Copyright 2009 Elsevier Ltd and European Society for Clinical Nutrition and 
      Metabolism. All rights reserved.
FAU - Aghazadeh, Safiyeh
AU  - Aghazadeh S
AD  - Institute of Biochemistry and Biophysics, P.O. Box 13145-1384, University of 
      Tehran, Tehran, Iran.
FAU - Yazdanparast, Razieh
AU  - Yazdanparast R
LA  - eng
PT  - Journal Article
DEP - 20090911
PL  - England
TA  - Clin Nutr
JT  - Clinical nutrition (Edinburgh, Scotland)
JID - 8309603
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Activators)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Plant Extracts)
RN  - 0 (teucrium extract)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/pharmacology/therapeutic use
MH  - Antioxidants/isolation & purification/pharmacology/therapeutic use
MH  - Caspase 3/metabolism
MH  - Enzyme Activation/drug effects
MH  - Enzyme Activators/isolation & purification/pharmacology/*therapeutic use
MH  - Enzyme Inhibitors/isolation & purification/pharmacology/*therapeutic use
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Fatty Liver/drug therapy/metabolism/pathology/*prevention & control
MH  - JNK Mitogen-Activated Protein Kinases/*antagonists & inhibitors/metabolism
MH  - Liver/drug effects/metabolism/pathology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphorylation/drug effects
MH  - *Phytotherapy
MH  - Plant Components, Aerial/chemistry
MH  - Plant Extracts/isolation & purification/pharmacology/therapeutic use
MH  - Rats
MH  - Teucrium/chemistry
EDAT- 2009/09/15 06:00
MHDA- 2010/09/30 06:00
CRDT- 2009/09/15 06:00
PHST- 2009/03/03 00:00 [received]
PHST- 2009/07/28 00:00 [revised]
PHST- 2009/08/14 00:00 [accepted]
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
AID - S0261-5614(09)00172-1 [pii]
AID - 10.1016/j.clnu.2009.08.014 [doi]
PST - ppublish
SO  - Clin Nutr. 2010 Jun;29(3):381-5. doi: 10.1016/j.clnu.2009.08.014. Epub 2009 Sep 
      11.