PMID- 19750196
OWN - NLM
STAT- MEDLINE
DCOM- 20100301
LR  - 20211020
IS  - 1528-3658 (Electronic)
IS  - 1076-1551 (Print)
IS  - 1076-1551 (Linking)
VI  - 15
IP  - 11-12
DP  - 2009 Nov-Dec
TI  - Platelet factor 4 is highly upregulated in dendritic cells after severe trauma.
PG  - 384-91
LID - 10.2119/molmed.2009.00074 [doi]
AB  - Dendritic cells (DCs) represent an important linkage between the innate and 
      adaptive immune system and express proinflammatory transcriptomic products early 
      after trauma. The use of a genomic approach recently revealed that platelet 
      factor 4 (PF4) is significantly upregulated in DCs in patients after multiple 
      trauma. However, knowledge about subsequent PF4 alteration and its potential 
      clinical relevance in the context of multiple trauma is still limited. We used 
      quantitative reverse transcription-polymerase chain reaction to analyze PF4 
      expression in both myeloid DCs (MDCs) and plasmocytoid DCs (PDCs) isolated from 
      10 patients after multiple trauma. Intracellular PF4 as well as HLA-DR expression 
      were detected by flow cytometry. Furthermore, DCs and peripheral blood 
      mononuclear cells were incubated on a monolayer of human umbilical endothelial 
      cells and their adhesion properties were analyzed. The ratio of the DC subtypes 
      (MDC and PDC) was assessed by flow cytometry. PF4 expression significantly 
      increased on d 1 and d 2 as measured by reverse transcription-polymerase chain 
      reaction. Intracellular PF4 content in MDCs and PDCs was significantly elevated 
      in trauma patients compared with healthy controls. In addition, the surface 
      antigen HLA-DR on MDCs was significantly elevated on d 1 and d 4 after trauma in 
      patients compared with controls. However, cell adhesion of DCs did not show any 
      significant differences between patients and controls. PF4 concentration in MDCs 
      and PDCs significantly correlated with the injury severity score. These results 
      confirm an early and subsequent posttraumatic activation of PF4 in DCs. PF4 also 
      participates in the posttraumatic activation of DCs in relation to injury 
      severity, a role that might be preferably based on the modification of receptor 
      expression, whereas adhesion properties are largely unaffected.
FAU - Maier, Marcus
AU  - Maier M
AD  - Department of Trauma, Hand and Reconstructive Surgery, Johann Wolfgang 
      Goethe-University, Frankfurt, Germany. Marcus.Maier@kgu.de
FAU - Geiger, Emanuel V
AU  - Geiger EV
FAU - Henrich, Dirk
AU  - Henrich D
FAU - Bendt, Carolyn
AU  - Bendt C
FAU - Wutzler, Sebastian
AU  - Wutzler S
FAU - Lehnert, Mark
AU  - Lehnert M
FAU - Marzi, Ingo
AU  - Marzi I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090827
PL  - England
TA  - Mol Med
JT  - Molecular medicine (Cambridge, Mass.)
JID - 9501023
RN  - 0 (HLA-DR Antigens)
RN  - 37270-94-3 (Platelet Factor 4)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism/*physiology
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression
MH  - HLA-DR Antigens/blood/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear
MH  - Male
MH  - Platelet Factor 4/genetics/*metabolism
MH  - Statistics, Nonparametric
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Trauma Severity Indices
MH  - Up-Regulation/drug effects
MH  - Wounds and Injuries/genetics/*metabolism
PMC - PMC2741291
EDAT- 2009/09/15 06:00
MHDA- 2010/03/02 06:00
PMCR- 2009/11/01
CRDT- 2009/09/15 06:00
PHST- 2009/06/14 00:00 [received]
PHST- 2009/08/26 00:00 [accepted]
PHST- 2009/09/15 06:00 [entrez]
PHST- 2009/09/15 06:00 [pubmed]
PHST- 2010/03/02 06:00 [medline]
PHST- 2009/11/01 00:00 [pmc-release]
AID - 09_74_maier [pii]
AID - 10.2119/molmed.2009.00074 [doi]
PST - ppublish
SO  - Mol Med. 2009 Nov-Dec;15(11-12):384-91. doi: 10.2119/molmed.2009.00074. Epub 2009 
      Aug 27.