PMID- 19751362
OWN - NLM
STAT- MEDLINE
DCOM- 20101110
LR  - 20100721
IS  - 1365-2605 (Electronic)
IS  - 0105-6263 (Linking)
VI  - 33
IP  - 4
DP  - 2010 Aug 1
TI  - Fertile bull sperm aneuploidy and chromatin integrity in relationship to 
      fertility.
PG  - 613-22
LID - 10.1111/j.1365-2605.2009.00989.x [doi]
AB  - Aneuploidy is associated with spontaneous abortions, perinatal mortality, mental 
      retardation and with embryonic and foetal mortality. Most of these abnormalities 
      originate as a result of meiosis errors during gametogenesis. The main purpose of 
      the study was to analyse frequency of aneuploidies of sex chromosomes and 
      chromosome 6 by three-colour fluorescence in situ hybridization (FISH) in 47 
      young bulls, candidates for artificial insemination programme with cryopreserved 
      semen and to investigate the influence of aneuploidies and disturbed sperm 
      chromatin integrity on non-return rates, the frequencies of abortions, perinatal 
      mortality and stillbirths. The average frequencies of spermatozoa with disomy for 
      chromosomes X, Y, XY and 6 were 0.032, 0.005, 0.003 and 0.039% respectively. The 
      incidence of XX66, YY66 and XY66 diploidy was 0.017, 0.006 and 0.015% 
      respectively. Frequencies of meiotic II errors were significantly higher than 
      meiotic I errors (p < 0.01). More X bearing spermatozoa than Y bearing 
      spermatozoa were detected (5151 vs. 5022; p < 0.01). Sperm chromatin damage 
      expressed by DNA fragmentation index (DFI) was 5.3 +/- 3.7 and percentage of 
      cells with defective chromatin condensation (HDS) was 1.4 +/- 0.8. No correlation 
      was found between sperm aneuploidy and basic sperm analysis. The relationship was 
      found between non-return rate and total aneuploidy (r = -0.310; p = 0.036). 
      Significant correlation was found between sex disomy, total aneuploidy (disomy of 
      chromosomes 6, X, Y and XY spermatozoa and diploidy) and stillbirths (r = 0.390; 
      p = 0.013; and r = 0.331; p = 0.037). Chromosome 6 disomy correlated with 
      perinatal mortality (r = 0.317; p = 0.047). HDS correlated significantly with 
      total aneuploidy (r = 0.449; p = 0.002). Our study indicated that aneuploidy 
      frequencies in young fertile bull spermatozoa are relatively low. Nevertheless, 
      there exists a variability in aneuploidy frequencies amongst bulls, which appears 
      to be able to have an influence on the fertility of these animals.
FAU - Rybar, R
AU  - Rybar R
AD  - Department of Genetics and Reproduction, Veterinary Research Institute, 621 00 
      Brno, Czech Republic. rybar@vri.cz
FAU - Kopecka, V
AU  - Kopecka V
FAU - Prinosilova, P
AU  - Prinosilova P
FAU - Kubickova, S
AU  - Kubickova S
FAU - Veznik, Z
AU  - Veznik Z
FAU - Rubes, J
AU  - Rubes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090720
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Chromatin)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Cattle/*genetics
MH  - Chromatin/*ultrastructure
MH  - DNA Fragmentation
MH  - Female
MH  - Fertility/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Semen Analysis/veterinary
MH  - Spermatozoa/*physiology
EDAT- 2009/09/16 06:00
MHDA- 2010/11/11 06:00
CRDT- 2009/09/16 06:00
PHST- 2009/09/16 06:00 [entrez]
PHST- 2009/09/16 06:00 [pubmed]
PHST- 2010/11/11 06:00 [medline]
AID - IJA989 [pii]
AID - 10.1111/j.1365-2605.2009.00989.x [doi]
PST - ppublish
SO  - Int J Androl. 2010 Aug 1;33(4):613-22. doi: 10.1111/j.1365-2605.2009.00989.x. 
      Epub 2009 Jul 20.