PMID- 19752036
OWN - NLM
STAT- MEDLINE
DCOM- 20091204
LR  - 20211020
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 77
IP  - 12
DP  - 2009 Dec
TI  - Th2 but not Th1 immune bias results in altered lung functions in a murine model 
      of pulmonary Cryptococcus neoformans infection.
PG  - 5389-99
LID - 10.1128/IAI.00809-09 [doi]
AB  - Changes in airway dynamics have been reported in the rat model of pulmonary 
      cryptococcosis. However, it is not known if Cryptococcus neoformans-induced 
      changes in lung functions are related to the immunophenotype that develops in 
      response to cryptococcal infection in the lungs. In this study we performed a 
      parallel analysis of the immunophenotype and airway resistance (standard 
      resistance of the airways [SRAW]) in BALB/c mice infected with highly virulent C. 
      neoformans strain H99 and moderately virulent strain 52D. H99 infection evoked a 
      Th2 response and was associated with increased SRAW, while the SRAW for 52D 
      infection, which resulted in a predominantly Th1-skewed response, did not differ 
      from the SRAW for uninfected mice. We found that an altered SRAW in mice did not 
      positively or negatively correlate with the pulmonary fungal burden, the 
      magnitude of inflammatory response, the numbers of T cells, eosinophils or 
      eosinophil subsets, neutrophils, or monocytes/macrophages, or the levels of 
      cytokines (interleukin-4 [IL-4], IL-10, gamma interferon, or IL-13) produced by 
      lung leukocytes. However, the level of a systemic Th2 marker, serum 
      immunoglobulin E (IgE), correlated significantly with SRAW, indicating that the 
      changes in lung functions were proportional to the level of Th2 skewing in this 
      model. These data also imply that IgE may contribute to the altered SRAW observed 
      in H99-infected mice. Lung histological analysis revealed severe allergic 
      bronchopulmonary mycosis pathology in H99-infected mice and evidence of 
      protective responses in 52D-infected mice with well-marginalized lesions. Taken 
      together, the data show that C. neoformans can significantly affect airflow 
      physiology, particularly in the context of a Th2 immune response with possible 
      involvement of IgE as an important factor.
FAU - Jain, Aditya V
AU  - Jain AV
AD  - Department of Veteran's Affairs Ann Arbor Healthcare System, Ann Arbor, Michigan 
      48105, USA.
FAU - Zhang, Yanmei
AU  - Zhang Y
FAU - Fields, W Bradley
AU  - Fields WB
FAU - McNamara, David A
AU  - McNamara DA
FAU - Choe, Mun Y
AU  - Choe MY
FAU - Chen, Gwo-Hsiao
AU  - Chen GH
FAU - Erb-Downward, John
AU  - Erb-Downward J
FAU - Osterholzer, John J
AU  - Osterholzer JJ
FAU - Toews, Galen B
AU  - Toews GB
FAU - Huffnagle, Gary B
AU  - Huffnagle GB
FAU - Olszewski, Michal A
AU  - Olszewski MA
LA  - eng
GR  - R01 AI059201/AI/NIAID NIH HHS/United States
GR  - R01 HL051082/HL/NHLBI NIH HHS/United States
GR  - R01-HL51082/HL/NHLBI NIH HHS/United States
GR  - R01-AI-059201/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090914
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Inflammation Mediators)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Brain/microbiology
MH  - Colony Count, Microbial
MH  - Cryptococcosis/*immunology/pathology
MH  - Cryptococcus neoformans/*immunology
MH  - Female
MH  - Immunoglobulin E/blood
MH  - Inflammation/pathology
MH  - Inflammation Mediators/analysis
MH  - Leukocytes/immunology
MH  - Lung/chemistry/microbiology/*pathology/*physiopathology
MH  - Lung Diseases, Fungal/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Respiratory Function Tests
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC2786439
EDAT- 2009/09/16 06:00
MHDA- 2009/12/16 06:00
PMCR- 2010/06/01
CRDT- 2009/09/16 06:00
PHST- 2009/09/16 06:00 [entrez]
PHST- 2009/09/16 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - IAI.00809-09 [pii]
AID - 0809-09 [pii]
AID - 10.1128/IAI.00809-09 [doi]
PST - ppublish
SO  - Infect Immun. 2009 Dec;77(12):5389-99. doi: 10.1128/IAI.00809-09. Epub 2009 Sep 
      14.