PMID- 19752600
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20090915
IS  - 1661-5433 (Electronic)
IS  - 1661-5425 (Linking)
VI  - 3
IP  - 4
DP  - 2009
TI  - Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 
      different breeds of cattle (Bos taurus).
PG  - 205-13
LID - 10.1159/000228721 [doi]
AB  - Multi-copied gene families are prevalent in mammalian genomes, especially within 
      the Y chromosome. Testis specific protein Y-encoded (TSPY) is present in variable 
      copy number in many mammalian species. Previous studies have estimated that TSPY 
      ranges from 50-200 copies in cattle. To examine TSPY localization on the Y 
      chromosome we employed fluorescence in situ hybridization (FISH) and fiber-FISH. 
      The results show a strong signal on the short arm of the Y chromosome (Yp). To 
      investigate TSPY copy number we used relative real-time polymerase chain reaction 
      (PCR) to analyze the DNA of 14 different cattle breeds. Variation both within and 
      between breeds was observed. All breeds show significant variation in TSPY copy 
      number among individual members. Brown Swiss (161 copies, CI = 133-195) had 
      higher average levels of TSPY and Western Fjord Cattle (63 copies, CI = 45-86) 
      had lower levels than some breeds. Overall, however, most breeds had a similar 
      average TSPY copy number. The pooled average was 94 copies (CI = 88-100). The 
      significance of the TSPY array remains uncertain, but as the function of TSPY is 
      unraveled the purpose of the array may become clearer.
CI  - (c) 2009 S. Karger AG, Basel.
FAU - Hamilton, C K
AU  - Hamilton CK
AD  - Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada.
FAU - Favetta, L A
AU  - Favetta LA
FAU - Di Meo, G P
AU  - Di Meo GP
FAU - Floriot, S
AU  - Floriot S
FAU - Perucatti, A
AU  - Perucatti A
FAU - Peippo, J
AU  - Peippo J
FAU - Kantanen, J
AU  - Kantanen J
FAU - Eggen, A
AU  - Eggen A
FAU - Iannuzzi, L
AU  - Iannuzzi L
FAU - King, W A
AU  - King WA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090911
PL  - Switzerland
TA  - Sex Dev
JT  - Sexual development : genetics, molecular biology, evolution, endocrinology, 
      embryology, and pathology of sex determination and differentiation
JID - 101316472
RN  - 0 (Cell Cycle Proteins)
SB  - IM
MH  - Animals
MH  - *Breeding
MH  - Cattle/*genetics
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - DNA Copy Number Variations/*genetics
MH  - Gene Dosage/*genetics
MH  - Gene Expression Regulation
MH  - Genome/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Organ Specificity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Testis/*metabolism
MH  - Y Chromosome/*genetics
EDAT- 2009/09/16 06:00
MHDA- 2010/01/22 06:00
CRDT- 2009/09/16 06:00
PHST- 2008/12/01 00:00 [received]
PHST- 2009/02/05 00:00 [accepted]
PHST- 2009/09/16 06:00 [entrez]
PHST- 2009/09/16 06:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
AID - 000228721 [pii]
AID - 10.1159/000228721 [doi]
PST - ppublish
SO  - Sex Dev. 2009;3(4):205-13. doi: 10.1159/000228721. Epub 2009 Sep 11.