PMID- 19754144
OWN - NLM
STAT- MEDLINE
DCOM- 20100128
LR  - 20131121
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 57
IP  - 19
DP  - 2009 Oct 14
TI  - Freeze-dried grape powder attenuates mitochondria- and oxidative stress-mediated 
      apoptosis in liver cells.
PG  - 9324-31
LID - 10.1021/jf900851n [doi]
AB  - The beneficial effects of grape consumption have been attributed to the 
      antioxidant activity of its polyphenols. This study was conducted to investigate 
      the cytoprotective effects of a freeze-dried grape powder (FDGP) on liver cells. 
      FDGP treatment of primary hepatocytes and hepatoma cells revealed increased 
      metabolic activity of cells and phosphorylation of Akt and IkappaBalpha, as well 
      as up-regulation of proliferating cell nuclear antigen (PCNA) level. To study the 
      molecular mechanisms of FDGP effects, cells were treated with TNF-related 
      apoptosis-inducing ligand (TRAIL); taurodeoxycholic acid (TDCA); thapsigargin 
      (TG), to induce cell apoptosis through death receptor-, mitochondria-, or 
      ER-mediated pathway; and H(2)O(2), to induce oxidative stress, respectively. 
      TDCA-induced activation of caspase-3, caspase-7, caspase-9, and Bax was 
      dramatically decreased with cotreatment of FDGP. Furthermore, FDGP reduced levels 
      of annexin V positive cells by 4-fold. Also, FDGP pretreatment restored cellular 
      glutathione content by 71% in cells treated with H(2)O(2). However, FDGP did not 
      inhibit ER-mediated apoptosis. In conclusion, FDGP increased the viability and 
      metabolic activity of liver cells and attenuated oxidative stress- and 
      mitochondria-mediated apoptosis. These data may contribute to the understanding 
      of the mechanisms involved in protective effects of grape in a variety of liver 
      conditions associated with cellular stress.
FAU - Xu, Yumin
AU  - Xu Y
AD  - Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
FAU - Khaoustov, Vladimir I
AU  - Khaoustov VI
FAU - Wang, Hui
AU  - Wang H
FAU - Yu, Jing
AU  - Yu J
FAU - Tabassam, Fazal
AU  - Tabassam F
FAU - Yoffe, Boris
AU  - Yoffe B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Polyphenols)
RN  - 516-50-7 (Taurodeoxycholic Acid)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular
MH  - Cell Line, Tumor
MH  - Flavonoids/analysis/pharmacology
MH  - *Food, Preserved
MH  - Freeze Drying
MH  - Fruit/chemistry
MH  - Hepatocytes/*drug effects
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Liver Neoplasms
MH  - Mice
MH  - Mitochondria, Liver/*physiology
MH  - Oxidative Stress/*drug effects
MH  - Phenols/analysis/pharmacology
MH  - Polyphenols
MH  - Taurodeoxycholic Acid/pharmacology
MH  - Vitis/*chemistry
EDAT- 2009/09/17 06:00
MHDA- 2010/01/29 06:00
CRDT- 2009/09/17 06:00
PHST- 2009/09/17 06:00 [entrez]
PHST- 2009/09/17 06:00 [pubmed]
PHST- 2010/01/29 06:00 [medline]
AID - 10.1021/jf900851n [doi]
PST - ppublish
SO  - J Agric Food Chem. 2009 Oct 14;57(19):9324-31. doi: 10.1021/jf900851n.