PMID- 19757043
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20211020
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 55
IP  - 7
DP  - 2010 Jul
TI  - Adipokines and cytokines in human pancreatic juice: unraveling the local 
      pancreatic inflammatory milieu.
PG  - 2108-12
LID - 10.1007/s10620-009-0977-z [doi]
AB  - BACKGROUND: Differential adipokine expression in obesity influences the 
      inflammatory milieu, and may explain in part obesity's negative impact on 
      pancreatic disease. Pancreatic juice analysis may provide a good means to 
      evaluate the local pancreatic inflammatory milieu. The presence of adipokines in 
      pancreatic juice is unknown. AIMS: This proof-of-concept study was designed to 
      determine the presence of adipokines and cytokines in human pancreatic juice. 
      METHODS: With institutional review board approval, pancreatic juice was obtained 
      from ten patients with a broad range of diagnoses at the time of endoscopic 
      retrograde cholangiopancreatography. Pancreatic juice was assayed using 
      enzyme-linked immunosorbent assay (ELISA) for insulin, the proinflammatory 
      adipokine leptin, the anti-inflammatory adipokine adiponectin, and the 
      inflammatory mediators interleukin-6 (IL-6), tumor necrosis factor-alpha 
      (TNF-alpha), and monocyte chemoattractant protein-1 (MCP-1). Correlation between 
      adipokine and inflammatory mediator expression was determined by linear 
      regression analysis. Data are presented as mean +/- standard error of the mean 
      (SEM); P < 0.05 was considered statistically significant. RESULTS: Leptin (0.16 
      +/- 0.05 ng/ml) and adiponectin (0.63 +/- 0.02 microg/ml) were both present, as 
      were the inflammatory mediators IL-6 (112.6 +/- 28.1 pg/ml), TNF-alpha (49.0 +/- 
      18.8 pg/ml), and MCP-1 (32.2 +/- 0.9 pg/ml). Paradoxically, the expression of the 
      anti-inflammatory adipokine adiponectin correlated strongly with that of the 
      proinflammatory cytokine IL-6 (R(2) = 0.98, P < 0.001). CONCLUSIONS: This report 
      is the first to describe adipokine expression in human pancreatic juice. Human 
      pancreatic juice inflammatory mediators and adipokines may provide an important 
      measurement of the local pancreatic inflammatory milieu.
FAU - Dalbec, Kathryn M
AU  - Dalbec KM
AD  - Department of Surgery, Indiana University School of Medicine, Indiana University, 
      Indianapolis, IN 46202, USA.
FAU - Max Schmidt, C
AU  - Max Schmidt C
FAU - Wade, Terence E
AU  - Wade TE
FAU - Wang, Sue
AU  - Wang S
FAU - Swartz-Basile, Deborah A
AU  - Swartz-Basile DA
FAU - Pitt, Henry A
AU  - Pitt HA
FAU - Zyromski, Nicholas J
AU  - Zyromski NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090916
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Adipokines)
RN  - 0 (Adiponectin)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Insulin)
RN  - 0 (Leptin)
SB  - IM
MH  - Adipokines/analysis/*metabolism
MH  - Adiponectin/analysis/metabolism
MH  - Adult
MH  - Aged
MH  - Cholangiopancreatography, Endoscopic Retrograde/methods
MH  - Cytokines/analysis/*metabolism
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Inflammation Mediators/analysis/*metabolism
MH  - Insulin/analysis/metabolism
MH  - Leptin/analysis/metabolism
MH  - Linear Models
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Function Tests
MH  - Pancreatic Juice/*chemistry
MH  - Pancreatitis/*diagnosis
MH  - Predictive Value of Tests
MH  - Probability
MH  - Prognosis
MH  - Risk Assessment
MH  - Sampling Studies
MH  - Severity of Illness Index
EDAT- 2009/09/17 06:00
MHDA- 2010/07/22 06:00
CRDT- 2009/09/17 06:00
PHST- 2009/04/16 00:00 [received]
PHST- 2009/08/31 00:00 [accepted]
PHST- 2009/09/17 06:00 [entrez]
PHST- 2009/09/17 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
AID - 10.1007/s10620-009-0977-z [doi]
PST - ppublish
SO  - Dig Dis Sci. 2010 Jul;55(7):2108-12. doi: 10.1007/s10620-009-0977-z. Epub 2009 
      Sep 16.