PMID- 19757044
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20211020
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 54
IP  - 12
DP  - 2009 Dec
TI  - A functional SNP of the Interleukin-18 gene is associated with the presence of 
      hepatocellular carcinoma in hepatitis B virus-infected patients.
PG  - 2722-8
LID - 10.1007/s10620-009-0970-6 [doi]
AB  - BACKGROUND/AIM: The natural course of hepatitis B virus (HBV) infection is likely 
      related to host immune factors. Interleukin-18 (IL-18) plays a significant role 
      in immune defense. This study was undertaken to determine the association between 
      the presence of hepatocellular carcinoma (HCC) and single-nucleotide 
      polymorphisms (SNPs) in the IL-18 gene in HBV-infected patients. METHODS: Between 
      March 2002 and December 2004, 730 Korean subjects were enrolled in two different 
      groups: (1) chronic carrier without HCC (n=637) and (2) HCC (n=93). We analyzed 
      SNPs at four polymorphic sites in the IL-18 gene at positions -667G>T, -148G>C, 
      +8925C>G, and +13925A>C in the study subjects. To evaluate the functional 
      significance of SNPs in the IL-18 gene promoter region, we performed a reporter 
      gene assay in HepG2 and Hep3B cells transfected with different alleles. RESULTS: 
      The IL-18 -148C allele, +8925G allele, +13925C allele, and haplotype 3 (TCGC) 
      were associated with the presence of HCC in codominant and dominant models. 
      Furthermore, functional analyses using the reporter gene assay revealed that the 
      -148C allele conferred significantly lower promoter activity. CONCLUSIONS: This 
      study indicates that the -148C, +8925G, and +13925C alleles of the IL-18 gene are 
      associated with the presence of HCC and the 148G>C SNP is functionally important 
      in determining disease outcome.
CI  - (c) Springer Science+Business Media, LLC 2009
FAU - Kim, Yong Seok
AU  - Kim YS
AD  - Department of Biochemistry and Molecular Biology, Hanyang University College of 
      Medicine, Seoul, South Korea.
FAU - Cheong, Jae Youn
AU  - Cheong JY
FAU - Cho, Sung Won
AU  - Cho SW
FAU - Lee, Kee Myung
AU  - Lee KM
FAU - Hwang, Jae Chul
AU  - Hwang JC
FAU - Oh, Bermseok
AU  - Oh B
FAU - Kimm, Kuchan
AU  - Kimm K
FAU - Lee, Jung A
AU  - Lee JA
FAU - Park, Byung Lae
AU  - Park BL
FAU - Cheong, Hyun Sub
AU  - Cheong HS
FAU - Shin, Hyoung Doo
AU  - Shin HD
FAU - Kim, Jin Hong
AU  - Kim JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090912
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Hepatocellular/*genetics/immunology/virology
MH  - Female
MH  - Gene Frequency
MH  - Genes, Reporter
MH  - Genetic Predisposition to Disease
MH  - Haplotypes
MH  - Hep G2 Cells
MH  - Hepatitis B, Chronic/*genetics/immunology
MH  - Humans
MH  - Interleukin-18/*genetics/metabolism
MH  - Linkage Disequilibrium
MH  - Liver Cirrhosis/genetics/immunology/virology
MH  - Liver Neoplasms/*genetics/immunology/virology
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Odds Ratio
MH  - Phenotype
MH  - *Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Prospective Studies
MH  - Republic of Korea
MH  - Risk Assessment
MH  - Risk Factors
MH  - Transfection
EDAT- 2009/09/17 06:00
MHDA- 2011/04/22 06:00
CRDT- 2009/09/17 06:00
PHST- 2009/06/17 00:00 [received]
PHST- 2009/08/24 00:00 [accepted]
PHST- 2009/09/17 06:00 [entrez]
PHST- 2009/09/17 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - 10.1007/s10620-009-0970-6 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2009 Dec;54(12):2722-8. doi: 10.1007/s10620-009-0970-6. Epub 2009 
      Sep 12.