PMID- 19757329
OWN - NLM
STAT- MEDLINE
DCOM- 20100311
LR  - 20161125
IS  - 1651-2065 (Electronic)
IS  - 0036-5599 (Linking)
VI  - 43
IP  - 6
DP  - 2009
TI  - Expression of renal aquaporins is down-regulated in children with congenital 
      hydronephrosis.
PG  - 486-93
LID - 10.3109/00365590903127446 [doi]
AB  - OBJECTIVE: This study investigated mRNA and protein expression of four renal 
      aquaporin isoforms [AQP1, 2, 3 and 4 (AQP1-4)] in congenital hydronephrotic 
      kidneys (HK) due to pelviureteral junction obstruction and normal control 
      kidneys. MATERIAL AND METHODS: The expression of AQP1-4 was evaluated in 22 
      children (15 boys, seven girls, aged 58.9+/-54.3 months) using semi-quantitative 
      reverse transcriptase-polymerase chain reaction (RT-PCR) techniques, 
      immunoblotting and immunohistochemistry. Hydronephrosis was graded by ultrasound 
      according to the Society for Fetal Urology. RESULTS: Both RT-PCR and 
      immunoblotting showed significantly reduced mRNA and protein expression of AQP1-4 
      in grade IV compared with those in grade III HK and controls. Sequencing 
      demonstrated 99% homology of AQP1-4 with those in GenBank. Positive 
      immunoreactivity of AQP1 was found in plasma membranes of the proximal tubule, 
      thin descending limb of Henle and descending vasa recta; AQP2 in the apical 
      membrane of collecting duct principal cells; and AQP3 and 4 in the basolateral 
      membrane of the same cells. In kidneys with grade IV hydronephrosis there was 
      reduction in the protein abundance of all four AQP isoforms which was more 
      pronounced compared with the protein abundance seen in kidneys with grade III 
      hydronephrosis and control kidneys. CONCLUSION: The expression of AQP1-4 mRNA and 
      protein abundance is reduced in proportion with the degree of hydronephrosis 
      graded by ultrasound in human congenital HK.
FAU - Wen, Jian Guo
AU  - Wen JG
AD  - Pediatric Urodynamic Center, Pediatric Surgery of First Affiliated Hospital of 
      Zhengzhou University, Institute of Clinical Medicine Universities Henan, PR 
      China. jgwen@zzu.edu.cn
FAU - Li, Zhen Zhen
AU  - Li ZZ
FAU - Zhang, Hong
AU  - Zhang H
FAU - Wang, Yan
AU  - Wang Y
FAU - Wang, Guixian
AU  - Wang G
FAU - Wang, Qingwei
AU  - Wang Q
FAU - Nielsen, Soren
AU  - Nielsen S
FAU - Djurhuus, Jens Christian
AU  - Djurhuus JC
FAU - Frokiaer, Jorgen
AU  - Frokiaer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Urol Nephrol
JT  - Scandinavian journal of urology and nephrology
JID - 0114501
RN  - 0 (Aquaporin 2)
RN  - 0 (Aquaporin 4)
RN  - 0 (Aquaporins)
RN  - 0 (RNA, Messenger)
RN  - 146410-94-8 (Aquaporin 1)
RN  - 158801-98-0 (Aquaporin 3)
SB  - IM
MH  - Aquaporin 1/metabolism
MH  - Aquaporin 2/metabolism
MH  - Aquaporin 3/metabolism
MH  - Aquaporin 4/metabolism
MH  - Aquaporins/*metabolism
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Down-Regulation/*physiology
MH  - Female
MH  - Humans
MH  - Hydronephrosis/*congenital/*metabolism/pathology
MH  - Infant
MH  - Kidney/diagnostic imaging/*metabolism/pathology
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Severity of Illness Index
MH  - Ultrasonography
EDAT- 2009/09/17 06:00
MHDA- 2010/03/12 06:00
CRDT- 2009/09/17 06:00
PHST- 2009/09/17 06:00 [entrez]
PHST- 2009/09/17 06:00 [pubmed]
PHST- 2010/03/12 06:00 [medline]
AID - 914939694 [pii]
AID - 10.3109/00365590903127446 [doi]
PST - ppublish
SO  - Scand J Urol Nephrol. 2009;43(6):486-93. doi: 10.3109/00365590903127446.