PMID- 1976117 OWN - NLM STAT- MEDLINE DCOM- 19901019 LR - 20190626 IS - 0910-5050 (Print) IS - 1876-4673 (Electronic) IS - 0910-5050 (Linking) VI - 81 IP - 6-7 DP - 1990 Jun-Jul TI - Acquired expression of hst-1 in an autonomous subline (Chiba subline 2) derived from androgen-responsive mouse mammary tumor (Shionogi carcinoma 115). PG - 554-6 AB - Since growth of Shionogi Carcinoma 115 (SC 115) and its autonomous subline (CS 2) were regulated by fibroblast growth factor-like peptide, expression of int-2 and hst-1 was examined in these cell lines. Hybridization of genomic DNA with long terminal repeat of mouse mammary tumor virus (MMTV) revealed the same pattern of restriction fragments, showing the same integration of MMTV. Although weak expression of int-2 was noticed in the two cells, clear expression of hst-1 was seen only in CS 2 cultured with/without testosterone. It is suggested that autonomous growth of androgen-unresponsive CS 2 is connected with expression of hst-1. FAU - Akakura, K AU - Akakura K AD - Department of Urology, School of Medicine, Chiba University. FAU - Furuya, Y AU - Furuya Y FAU - Sato, N AU - Sato N FAU - Kodama, T AU - Kodama T FAU - Teshima, S AU - Teshima S FAU - Shimosato, Y AU - Shimosato Y FAU - Yoshida, T AU - Yoshida T FAU - Terada, M AU - Terada M FAU - Shimazaki, J AU - Shimazaki J LA - eng PT - Journal Article PL - Japan TA - Jpn J Cancer Res JT - Japanese journal of cancer research : Gann JID - 8509412 RN - 3XMK78S47O (Testosterone) RN - 62031-54-3 (Fibroblast Growth Factors) RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - Blotting, Southern MH - Cells, Cultured MH - DNA/analysis MH - Fibroblast Growth Factors/*biosynthesis MH - *Gene Expression Regulation, Neoplastic MH - In Vitro Techniques MH - Mammary Neoplasms, Animal/*metabolism MH - Mice MH - Nucleic Acid Hybridization MH - Polymorphism, Restriction Fragment Length MH - Testosterone/pharmacology PMC - PMC6504066 EDAT- 1990/06/01 00:00 MHDA- 1990/06/01 00:01 PMCR- 1990/06/01 CRDT- 1990/06/01 00:00 PHST- 1990/06/01 00:00 [pubmed] PHST- 1990/06/01 00:01 [medline] PHST- 1990/06/01 00:00 [entrez] PHST- 1990/06/01 00:00 [pmc-release] AID - CAE554 [pii] AID - 10.1111/j.1349-7006.1990.tb02606.x [doi] PST - ppublish SO - Jpn J Cancer Res. 1990 Jun-Jul;81(6-7):554-6. doi: 10.1111/j.1349-7006.1990.tb02606.x.