PMID- 1976120
OWN - NLM
STAT- MEDLINE
DCOM- 19901019
LR  - 20231011
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 81
IP  - 6-7
DP  - 1990 Jun-Jul
TI  - A congenic line of the DDD mouse strain, DDD/1-Mtv-2/Mtv-2: establishment and 
      mammary tumorigenesis.
PG  - 639-44
AB  - A single dominant gene on chromosome 18, Mtv-2, controls both the early 
      appearance of mammary tumors and expression of mouse mammary tumor virus (MMTV) 
      in the milk. A congenic DDD mouse strain, DDD/1-Mtv-2/Mtv-2 (DDD-Mtv-2), was 
      developed by introducing this gene from GRS/AJms (GR) into DDD/1 mice by 
      repeating 12 backcrosses and subsequent inbreeding using mammary tumors as a 
      marker for selection. Southern blot analysis of the liver DNA from the resulting 
      congenic mice with EcoRI and MMTV-U3 prove revealed that two DNA fragments 
      corresponding to Mtv-2 were specifically transferred from GR to congenic mice. 
      Detection of MMTV-gp52 antigen in the mammary gland and mammary tumor development 
      in DDDfDDD-Mtv-2 mice demonstrated the production of infectious mature MMTV by 
      Mtv-2 in congenic mice. About 80% of breeding DDD-Mtv-2 females developed mammary 
      tumors in the course of one-year follow-up. The tumor incidence was lower and the 
      tumor age higher than those in GR mice, suggesting less active functioning of the 
      gene on the DDD genetic background. About 70% of these tumors were 
      morphologically classified as pale cell and type P carcinomas peculiar to GR 
      mice. The gene seemed to control the histologic features of mammary tumors. 
      Congenic mice carried an MMTV provirus in an incomplete form on Y chromosome. The 
      DDD-Mtv-2-strain will provide a new model for biological and molecular researches 
      into mouse mammary tumorigenesis.
FAU - Matsuzawa, A
AU  - Matsuzawa A
AD  - Laboratory Animal Research Center, Institute of Medical Science, University of 
      Tokyo.
FAU - Sayama, K
AU  - Sayama K
FAU - Tsubura, A
AU  - Tsubura A
FAU - Murakami, A
AU  - Murakami A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Crosses, Genetic
MH  - DNA/analysis
MH  - Female
MH  - Gene Expression
MH  - Liver/analysis
MH  - Male
MH  - Mammary Neoplasms, Experimental/*genetics/pathology
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Polymorphism, Restriction Fragment Length
PMC - PMC6504069
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
PMCR- 1990/06/01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PHST- 1990/06/01 00:00 [pmc-release]
AID - CAE639 [pii]
AID - 10.1111/j.1349-7006.1990.tb02621.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1990 Jun-Jul;81(6-7):639-44. doi: 
      10.1111/j.1349-7006.1990.tb02621.x.