PMID- 19762030
OWN - NLM
STAT- MEDLINE
DCOM- 20091214
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 45
DP  - 2009 Nov 6
TI  - Validation of an ultra high pressure liquid chromatographic method for the 
      determination of biologically active amines in food.
PG  - 7715-20
LID - 10.1016/j.chroma.2009.08.072 [doi]
AB  - Biologically active amines include the so called biogenic amines, such as 
      histamine, tyramine and cadaverine, and polyamines such as spermidine and 
      spermine. Ultra high pressure liquid chromatography (UHPLC) is a new generation 
      of separation techniques that takes full advantage of chromatographic principles 
      to increase speed flow which drastically reduce analysis time. The aim of the 
      present work was to validate a rapid method of UHPLC to detect the presence of 
      biogenic amines and polyamines in food. Different food matrixes (wine, fish, 
      cheese, and dry fermented sausage) were used in order to test the versatility of 
      the method. The UHPLC method described in this article has been demonstrated as a 
      reliable procedure to determine 12 biogenic amines and polyamines in less than 
      7min of chromatographic elution. The method provides a satisfactory linearity and 
      chromatographic sensitivity with a detection limit lower than 0.2mg/L and a 
      determination limit falling below 0.3mg/L for all amines. The precision, in terms 
      of relative standard deviation, was lower than 5% and the accuracy, as mean 
      recovery, was between 93% and 98%, depending on the food matrix.
FAU - Latorre-Moratalla, M L
AU  - Latorre-Moratalla ML
AD  - Department of Nutrition and Food Science, XaRTA (Catalonian Reference Network on 
      Food Technology) - INSA (Nutrition and Food Safety Research Institute), UB, Av. 
      Joan XXIII s/n, E-08028, Barcelona, Spain.
FAU - Bosch-Fuste, J
AU  - Bosch-Fuste J
FAU - Lavizzari, T
AU  - Lavizzari T
FAU - Bover-Cid, S
AU  - Bover-Cid S
FAU - Veciana-Nogues, M T
AU  - Veciana-Nogues MT
FAU - Vidal-Carou, M C
AU  - Vidal-Carou MC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090901
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Biogenic Amines)
SB  - IM
MH  - Animals
MH  - Biogenic Amines/*analysis
MH  - Cheese/analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fishes
MH  - Food Analysis/*methods
MH  - Meat Products/analysis
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Wine/analysis
EDAT- 2009/09/19 06:00
MHDA- 2009/12/16 06:00
CRDT- 2009/09/19 06:00
PHST- 2009/04/22 00:00 [received]
PHST- 2009/08/23 00:00 [revised]
PHST- 2009/08/27 00:00 [accepted]
PHST- 2009/09/19 06:00 [entrez]
PHST- 2009/09/19 06:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0021-9673(09)01305-3 [pii]
AID - 10.1016/j.chroma.2009.08.072 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 Nov 6;1216(45):7715-20. doi: 10.1016/j.chroma.2009.08.072. 
      Epub 2009 Sep 1.