PMID- 19762528
OWN - NLM
STAT- MEDLINE
DCOM- 20091001
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 132
IP  - 4
DP  - 2009 Oct
TI  - Chromogenic in situ hybridization: a multicenter study comparing silver in situ 
      hybridization with FISH.
PG  - 514-20
LID - 10.1309/AJCPXY3MJ6GSRCYP [doi]
AB  - Our purposes were to perform a robust assessment of a new HER2 chromogenic in 
      situ hybridization test and report on concordance of silver in situ hybridization 
      (SISH) data with fluorescence in situ hybridization (FISH) data and on 
      intraobserver and interlaboratory scoring consistency. HER2 results were scored 
      from 45 breast cancers in 7 laboratories using the Ventana (Tucson, AZ) INFORM 
      HER-2 SISH assay and in 1 central laboratory using a standard FISH assay. 
      Overall, 94.8% of cases were successfully analyzed by SISH across the 6 
      participating laboratories that reported data. Concordance for diagnosis of HER2 
      amplification by SISH compared with FISH was high (96.0% overall). Intraobserver 
      variability (8.0%) and intersite variability (12.66%) of absolute HER2/chromosome 
      17 ratios appear to be tightly controlled across all 6 participating 
      laboratories. The Ventana INFORM HER-2 SISH assay is robust and reproducible, 
      shows good concordance with a standard FISH assay, and complies with requirements 
      in national guidelines for performance of diagnostic tests.
FAU - Bartlett, J M S
AU  - Bartlett JM
AD  - Endocrine Cancer Group, Edinburgh University Cancer Research Centre, Western 
      General Hospital, Edinburgh EH4 2XR, England.
FAU - Campbell, Fiona M
AU  - Campbell FM
FAU - Ibrahim, Merdol
AU  - Ibrahim M
FAU - Wencyk, Peter
AU  - Wencyk P
FAU - Ellis, Ian
AU  - Ellis I
FAU - Kay, Elaine
AU  - Kay E
FAU - Connolly, Yvonne
AU  - Connolly Y
FAU - O'Grady, Anthony
AU  - O'Grady A
FAU - Di Palma, Silvana
AU  - Di Palma S
FAU - Starczynski, Jane
AU  - Starczynski J
FAU - Morgan, John M
AU  - Morgan JM
FAU - Jasani, Bharat
AU  - Jasani B
FAU - Miller, Keith
AU  - Miller K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 3M4G523W1G (Silver)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/diagnosis
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - *Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Observer Variation
MH  - Receptor, ErbB-2/analysis
MH  - Reproducibility of Results
MH  - Silver
EDAT- 2009/09/19 06:00
MHDA- 2009/10/02 06:00
CRDT- 2009/09/19 06:00
PHST- 2009/09/19 06:00 [entrez]
PHST- 2009/09/19 06:00 [pubmed]
PHST- 2009/10/02 06:00 [medline]
AID - 132/4/514 [pii]
AID - 10.1309/AJCPXY3MJ6GSRCYP [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2009 Oct;132(4):514-20. doi: 10.1309/AJCPXY3MJ6GSRCYP.