PMID- 19765465
OWN - NLM
STAT- MEDLINE
DCOM- 20100111
LR  - 20090921
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 41
IP  - 7
DP  - 2009 Sep
TI  - Apoptosis and proliferation of cardiomyocytes and interstitial mononuclear cells: 
      association with rejection and macrophage infiltration.
PG  - 2890-2
LID - 10.1016/j.transproceed.2009.07.031 [doi]
AB  - Previous studies have noted that acute rejection episodes (AREs) may lead to loss 
      of cardiomyocytes in transplanted hearts. The aim of this study was twofold; 
      first, to assess the degree of apoptotic cells and to compute the proliferation 
      index of cardiomyocytes and mononuclear interstitial infiltrates in cardiac 
      allografts. Second, we sought to determine whether apoptosis involved in AREs was 
      associated with macrophage infiltration. Among 28 endomyocardial biopsies, 18 
      showed AREs and the remaining 10 biopsies, nonspecific changes, the control 
      group. All biopsies were immunostained with Ki-67 and CD68 antibodies. Apoptotic 
      cells were counted using the terminal deoxyncleotidyl transferase dUTP nick end 
      labeling method. Apoptotic death of cardiac myocytes and interstitial mononuclear 
      cells was significantly greater in cases of allograft rejection compared with the 
      control group (P < .05). In addition, compared to the control group, ARE cases 
      showed a greater proliferation index of cardiac myocytes and interstitial cells 
      (P < .05). Macrophage infiltration was significantly greater in ARE cases and 
      macrophage infiltration showed a linear association with both apoptosis and 
      proliferation of myocytes and interstitial cells (P < .001). In conclusion, we 
      verified the presence of apoptotic cell death during acute rejection in heart 
      transplants. Apoptotic cell death was significant among interstitial cells but it 
      was less among cardiac myocytes. Macrophage infiltration had a great influence on 
      apoptotic cell death of myocytes and interstitial cells.
FAU - Ozdemir, B H
AU  - Ozdemir BH
AD  - Pathology Department, Baskent University, Faculty of Medicine, Ankara, Turkey. 
      handan27@hotmail.com
FAU - Sezgin, A
AU  - Sezgin A
FAU - Haberal, M
AU  - Haberal M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
SB  - IM
MH  - Apoptosis
MH  - Biopsy
MH  - Cell Death
MH  - Cell Division
MH  - Graft Rejection/*pathology/physiopathology
MH  - Heart Transplantation/adverse effects/*pathology
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/pathology/physiology
MH  - Macrophages/*pathology
MH  - Myocytes, Cardiac/cytology/pathology/physiology
EDAT- 2009/09/22 06:00
MHDA- 2010/01/12 06:00
CRDT- 2009/09/22 06:00
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/01/12 06:00 [medline]
AID - S0041-1345(09)01066-5 [pii]
AID - 10.1016/j.transproceed.2009.07.031 [doi]
PST - ppublish
SO  - Transplant Proc. 2009 Sep;41(7):2890-2. doi: 10.1016/j.transproceed.2009.07.031.