PMID- 19765786
OWN - NLM
STAT- MEDLINE
DCOM- 20100427
LR  - 20091201
IS  - 1873-3700 (Electronic)
IS  - 0031-9422 (Linking)
VI  - 70
IP  - 17-18
DP  - 2009 Dec
TI  - Methyl angolensate changes in Khaya ivorensis after fungal infection.
PG  - 2027-33
LID - 10.1016/j.phytochem.2009.08.013 [doi]
AB  - Khaya ivorensis with and without symptoms of stem and branch cankers, caused by 
      Botryosphaeria rhodina were examined in order to determine whether the secondary 
      metabolites in this plant were associated with a chemical defense response. This 
      study provides evidence that the limonoid methyl angolensate (MA) is present at 
      higher concentrations in K. ivorensis with symptoms of stem cankers rather than 
      in the plants without symptoms. A rapid, sensitive and selective HPLC-ESI-MS/MS 
      method (using selected reaction monitoring--SRM--mode) was developed for 
      quantification of MA in all aerials parts of such plants, with a good linearity 
      over a range of 0.1-20.0 g/kg, with r(2)>0.996+/-6.1%. The limits of detection 
      (LOD) and quantification (LOQ) were less than 0.03 g/kg and 0.08 g/kg, 
      respectively. Relative Standard Deviations (RSDs) ranged from 1.7% to 19.2% for 
      all matrices. While the MA concentration did not change in the stem bark, its 
      amounts increased nearly fourfold in stems and by 20% in leaves, when plants with 
      symptoms were compared with those without symptoms. These data suggest that MA 
      plays a role in plant-pathogen interactions, probably as a phytoanticipin.
FAU - Reche, Karine V G
AU  - Reche KV
AD  - Laboratorio de Produtos Naturais, Departamento de Quimica, UFSCar. Rodovia 
      Washington Luiz, Km 235, Postcode 676, CEP 13565-905, Sao Carlos, Sao Paulo, 
      Brazil.
FAU - de Souza, Gezimar D
AU  - de Souza GD
FAU - Trapp, Marilia A
AU  - Trapp MA
FAU - Rodrigues-Filho, Edson
AU  - Rodrigues-Filho E
FAU - Silva, Sebastiao C
AU  - Silva SC
FAU - Fernandes, Joao B
AU  - Fernandes JB
FAU - Vieira, Paulo C
AU  - Vieira PC
FAU - Muller, Manfred W
AU  - Muller MW
FAU - da Silva, M Fatima das G F
AU  - da Silva MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090916
PL  - England
TA  - Phytochemistry
JT  - Phytochemistry
JID - 0151434
RN  - 0 (Triterpenes)
RN  - 2629-14-3 (methyl angolensate)
SB  - IM
MH  - Fungi/*pathogenicity
MH  - *Host-Pathogen Interactions
MH  - Meliaceae/*metabolism/microbiology
MH  - *Plant Diseases
MH  - Plant Structures
MH  - Triterpenes/*metabolism
EDAT- 2009/09/22 06:00
MHDA- 2010/04/28 06:00
CRDT- 2009/09/22 06:00
PHST- 2008/10/07 00:00 [received]
PHST- 2009/07/28 00:00 [revised]
PHST- 2009/08/18 00:00 [accepted]
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/04/28 06:00 [medline]
AID - S0031-9422(09)00365-3 [pii]
AID - 10.1016/j.phytochem.2009.08.013 [doi]
PST - ppublish
SO  - Phytochemistry. 2009 Dec;70(17-18):2027-33. doi: 10.1016/j.phytochem.2009.08.013. 
      Epub 2009 Sep 16.