PMID- 19766400
OWN - NLM
STAT- MEDLINE
DCOM- 20101223
LR  - 20161125
IS  - 1879-1409 (Electronic)
IS  - 0305-4179 (Linking)
VI  - 36
IP  - 4
DP  - 2010 Jun
TI  - Is HBOT treatment effective in recovering zone of stasis? An experimental 
      immunohistochemical study.
PG  - 539-44
LID - 10.1016/j.burns.2009.06.210 [doi]
AB  - Studies aimed at recovering the zone of stasis are one of the major issues of 
      experimental burn studies. Hypoxia and oedema at that zone may cause irreversible 
      changes. Due to anti-oedematous and antihypoxic effects of hyperbaric oxygen 
      treatment (HBOT), it may be beneficial in recovering the zone of stasis. We 
      performed an experimental study using 20 Sprague-Dawley rats, each weighing 
      350-450 g. The rats were first divided into two groups as 24h and 5 days. 
      Subsequently, control and treatment groups (five rats in each group) were formed. 
      For burn wounds, we used the burn comb model, which was described by Regas and 
      Ehrlich. In the treatment group, 2.5 ATA HBOT was applied for 90 min twice daily. 
      A 0.8-cm punch biopsy was performed and samples for histological examination were 
      taken from the centre of burn area. The rats were sacrificed by administering '3 
      mci technetium-99m methoxy butyl nitrite' ((99)Tc(m) MIBI) through the femoral 
      vein. Biopsy materials were evaluated by histological and immunohistochemical 
      methods. Dorsal skin fragment, excised to 1cm margin, was imaged by scintigraphic 
      measurements with a gamma camera. Wet and dry weight measurements of excised skin 
      fragments were taken. As a result, HBOT showed a positive effect at the cellular 
      level in the first 24h. It increased recovery potential by augmenting 
      neovascularisation and decreasing oedema in the 5-day group.
CI  - (c) 2009 Elsevier Ltd and ISBI. All rights reserved.
FAU - Turkaslan, Tayfun
AU  - Turkaslan T
AD  - Department of Plastic and Reconstructive Surgery, Vakif Gureba Training and 
      Research Hospital, Istanbul, Turkey. tturkaslan@hotmail.com
FAU - Yogun, Nilay
AU  - Yogun N
FAU - Cimsit, Maide
AU  - Cimsit M
FAU - Solakoglu, Seyhun
AU  - Solakoglu S
FAU - Ozdemir, Cuneyt
AU  - Ozdemir C
FAU - Ozsoy, Zafer
AU  - Ozsoy Z
LA  - eng
PT  - Journal Article
DEP - 20090918
PL  - Netherlands
TA  - Burns
JT  - Burns : journal of the International Society for Burn Injuries
JID - 8913178
SB  - IM
MH  - Animals
MH  - Biopsy
MH  - Burns/pathology/*therapy
MH  - Disease Models, Animal
MH  - Edema/pathology/prevention & control
MH  - *Hyperbaric Oxygenation
MH  - Hypoxia/pathology/prevention & control
MH  - Immunohistochemistry
MH  - Necrosis/prevention & control
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skin/*blood supply/injuries
MH  - Wound Healing/physiology
EDAT- 2009/09/22 06:00
MHDA- 2010/12/25 06:00
CRDT- 2009/09/22 06:00
PHST- 2008/11/28 00:00 [received]
PHST- 2009/06/11 00:00 [revised]
PHST- 2009/06/24 00:00 [accepted]
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/12/25 06:00 [medline]
AID - S0305-4179(09)00397-0 [pii]
AID - 10.1016/j.burns.2009.06.210 [doi]
PST - ppublish
SO  - Burns. 2010 Jun;36(4):539-44. doi: 10.1016/j.burns.2009.06.210. Epub 2009 Sep 18.