PMID- 19766485
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20220408
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 101
IP  - 3
DP  - 2010 Feb
TI  - Identification of fungi associated with municipal compost using DNA-based 
      techniques.
PG  - 1021-7
LID - 10.1016/j.biortech.2009.08.109 [doi]
AB  - Fungi are important in terrestrial decay processes. However, fungi associated 
      with organic decay during composting are still not well known. In this study 
      culture-independent methods were used to identify fungi associated with 
      composting organic municipal wastes to gain a better understanding of the 
      diversity of fungi associated with this process. Fungal communities from 0, 210, 
      and 410day-old compost samples were assessed with DNA fingerprinting using 
      denaturing gradient gel electrophoresis (DGGE) and by the analysis of DNA 
      sequences from rDNA clone libraries. From 207 rDNA sequences, 82 fungal OTU's 
      were detected. A disproportionate number of yeast sequences were detected in Day 
      0 clone libraries, including the human pathogens Candida tropicalis and Candida 
      krusei (Saccharomycetales). Basidiomycetes accounted for over half of the clones 
      from the Day 210 sample. Clones of Cercophora and Neurospora species accounted 
      for most of the fungal clones of the Day 410 sample. No Zygomycetes or 
      Aspergillus species were detected in this study. These findings call for a 
      reassessment of long held views about the organisms involved in the composting of 
      organic municipal wastes.
FAU - Bonito, Gregory
AU  - Bonito G
AD  - Biology Department, Duke University, Durham, NC 27708, United States. 
      Gregory.Bonito@duke.edu
FAU - Isikhuemhen, Omoanghe S
AU  - Isikhuemhen OS
FAU - Vilgalys, Rytas
AU  - Vilgalys R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090918
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Soil)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aspergillus/metabolism
MH  - Candida/*metabolism
MH  - DNA/*chemistry
MH  - DNA, Fungal/genetics
MH  - DNA, Ribosomal/chemistry
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Fungi/*metabolism
MH  - Humans
MH  - Neurospora/metabolism
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - *Soil
MH  - Soil Microbiology
MH  - Temperature
MH  - Time Factors
EDAT- 2009/09/22 06:00
MHDA- 2010/02/18 06:00
CRDT- 2009/09/22 06:00
PHST- 2009/02/17 00:00 [received]
PHST- 2009/08/28 00:00 [revised]
PHST- 2009/08/28 00:00 [accepted]
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - S0960-8524(09)01175-4 [pii]
AID - 10.1016/j.biortech.2009.08.109 [doi]
PST - ppublish
SO  - Bioresour Technol. 2010 Feb;101(3):1021-7. doi: 10.1016/j.biortech.2009.08.109. 
      Epub 2009 Sep 18.