PMID- 19766576
OWN - NLM
STAT- MEDLINE
DCOM- 20100122
LR  - 20091201
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 27
IP  - 52
DP  - 2009 Dec 9
TI  - Influenza recombinant vaccine: matrix protein M1 on the platform of the 
      adenovirus dodecahedron.
PG  - 7385-93
LID - 10.1016/j.vaccine.2009.09.021 [doi]
AB  - We propose a novel influenza vaccine composed of the adenovirus dodecahedron (Dd) 
      as delivery platform carrying an internal influenza matrix protein M1. To attach 
      the antigen to the vector we used WW domains interacting with Dd. Successful 
      internalization of the Dd-M1WW complex was observed using biochemical and cell 
      biology techniques. We show here that the complex of Dd with antigen is a potent 
      activator of human myeloid dendritic cells (MDC), and that it is efficiently 
      presented by MDC to M1-specific CD8+ T lymphocytes. These results show that 
      proposed vaccine model is feasible and that adenovirus dodecahedron is a potent 
      delivery platform for foreign antigens to human cells.
FAU - Naskalska, A
AU  - Naskalska A
AD  - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. 
      Pawinskiego 5a, 02-106 Warsaw, Poland.
FAU - Szolajska, E
AU  - Szolajska E
FAU - Chaperot, L
AU  - Chaperot L
FAU - Angel, J
AU  - Angel J
FAU - Plumas, J
AU  - Plumas J
FAU - Chroboczek, J
AU  - Chroboczek J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090918
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Influenza Vaccines)
RN  - 0 (M1 protein, Influenza A virus)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Adenoviridae/*immunology
MH  - Amino Acid Sequence
MH  - Antigen Presentation/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cloning, Molecular
MH  - Dendritic Cells/immunology
MH  - HeLa Cells
MH  - Humans
MH  - Influenza A Virus, H1N1 Subtype/immunology
MH  - Influenza Vaccines/biosynthesis/*immunology
MH  - Influenza, Human/prevention & control
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/immunology
MH  - Vaccines, Synthetic/immunology
MH  - Viral Matrix Proteins/*immunology
EDAT- 2009/09/22 06:00
MHDA- 2010/01/23 06:00
CRDT- 2009/09/22 06:00
PHST- 2009/04/24 00:00 [received]
PHST- 2009/09/01 00:00 [revised]
PHST- 2009/09/06 00:00 [accepted]
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/01/23 06:00 [medline]
AID - S0264-410X(09)01345-0 [pii]
AID - 10.1016/j.vaccine.2009.09.021 [doi]
PST - ppublish
SO  - Vaccine. 2009 Dec 9;27(52):7385-93. doi: 10.1016/j.vaccine.2009.09.021. Epub 2009 
      Sep 18.