PMID- 19767042
OWN - NLM
STAT- MEDLINE
DCOM- 20100602
LR  - 20100226
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 88
IP  - 2
DP  - 2010 Apr
TI  - Localization of porcine reproductive and respiratory syndrome virus in mammary 
      glands of experimentally infected sows.
PG  - 304-6
LID - 10.1016/j.rvsc.2009.08.008 [doi]
AB  - We investigated the localization of porcine reproductive and respiratory syndrome 
      virus (PRRSV) in the mammary glands of experimentally infected sows using reverse 
      transcription PCR and immunohistochemistry. Six pregnant sows were inoculated 
      intranasally with PRRSV three weeks prior to the predicted farrowing date. The 
      six PRRSV-infected sows and two uninfected negative control sows remained 
      clinically healthy and farrowed normally. Milk samples were collected from all 
      sows on lactation days 1, 2, and 3. PRRSV was detected in milk as early as 
      lactation day 1 in all of the PRRSV-infected sows, but not in the uninfected 
      sows. PRRSV antigen was detected by immunohistochemistry in macrophage-like cells 
      in the alveolar lumina of the mammary glands and in other tissues. Our results 
      show that PRRSV is shed in the milk of infected sows, and that PRRSV antigen is 
      present in the mammary glands of experimentally infected sows.
CI  - Copyright 2009 Elsevier Ltd. All rights reserved.
FAU - Kang, Ikjae
AU  - Kang I
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic 
      of Korea.
FAU - Ha, Yooncheol
AU  - Ha Y
FAU - Kim, Duyeol
AU  - Kim D
FAU - Oh, Yeonsu
AU  - Oh Y
FAU - Cho, Kyung-Dong
AU  - Cho KD
FAU - Lee, Bog-Hieu
AU  - Lee BH
FAU - Lim, Junghan
AU  - Lim J
FAU - Kim, Sung-Hoon
AU  - Kim SH
FAU - Kwon, Byungjoon
AU  - Kwon B
FAU - Chae, Chanhee
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090919
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
SB  - IM
MH  - Animals
MH  - Female
MH  - Immunohistochemistry
MH  - Infectious Disease Transmission, Vertical/veterinary
MH  - Lactation
MH  - Mammary Glands, Animal/*virology
MH  - Milk/virology
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Pregnancy
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Swine Diseases/*virology
EDAT- 2009/09/22 06:00
MHDA- 2010/06/03 06:00
CRDT- 2009/09/22 06:00
PHST- 2008/11/25 00:00 [received]
PHST- 2009/07/11 00:00 [revised]
PHST- 2009/08/20 00:00 [accepted]
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2010/06/03 06:00 [medline]
AID - S0034-5288(09)00213-6 [pii]
AID - 10.1016/j.rvsc.2009.08.008 [doi]
PST - ppublish
SO  - Res Vet Sci. 2010 Apr;88(2):304-6. doi: 10.1016/j.rvsc.2009.08.008. Epub 2009 Sep 
      19.