PMID- 19767511
OWN - NLM
STAT- MEDLINE
DCOM- 20091222
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 114
IP  - 20
DP  - 2009 Nov 12
TI  - Homeostasis of dendritic cells in lymphoid organs is controlled by regulation of 
      their precursors via a feedback loop.
PG  - 4411-21
LID - 10.1182/blood-2008-11-188045 [doi]
AB  - Dendritic cells (DCs) are key coordinators of the immune response, governing the 
      choice between tolerance and immunity. Despite their importance, the mechanisms 
      controlling the size of the DC compartment are largely unknown. Using a mouse 
      model allowing continuous DC depletion, we show that maintenance of DC numbers in 
      spleen is an active process mediated by Flt3-L-dependent regulation of precursor 
      differentiation into DCs, rather than by changes in proliferation of the 
      differentiated DCs. In particular, the frequency and differentiation potential of 
      intrasplenic DC precursors increased in response to reduced DC numbers. Levels of 
      Flt3-L, a cytokine required for DC differentiation, increased in the blood after 
      DC depletion and returned to normal levels once the DC compartment filled up 
      again. Our data suggest a feedback regulation of DC homeostasis whereby reduction 
      of the DC pool size promotes differentiation of their precursors, via increased 
      Flt3-L availability. This mechanism is different to those known for other immune 
      cell types, such as the B- and T-cell compartments, whereby lymphopenia induces 
      proliferation of already differentiated lymphocytes.
FAU - Hochweller, Kristin
AU  - Hochweller K
AD  - Division of Molecular Immunology, German Cancer Research Center (DKFZ), 
      Heidelberg, Germany.
FAU - Miloud, Tewfik
AU  - Miloud T
FAU - Striegler, Jorg
AU  - Striegler J
FAU - Naik, Shalin
AU  - Naik S
FAU - Hammerling, Gunter J
AU  - Hammerling GJ
FAU - Garbi, Natalio
AU  - Garbi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090918
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - EC 2.7.10.1 (Flt3 protein, mouse)
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Cell Differentiation/*immunology
MH  - Dendritic Cells/*cytology/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Feedback, Physiological/*physiology
MH  - Flow Cytometry
MH  - Homeostasis/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/cytology/immunology
MH  - Stem Cells/*cytology/immunology
MH  - fms-Like Tyrosine Kinase 3
EDAT- 2009/09/22 06:00
MHDA- 2009/12/23 06:00
CRDT- 2009/09/22 06:00
PHST- 2009/09/22 06:00 [entrez]
PHST- 2009/09/22 06:00 [pubmed]
PHST- 2009/12/23 06:00 [medline]
AID - S0006-4971(20)38923-0 [pii]
AID - 10.1182/blood-2008-11-188045 [doi]
PST - ppublish
SO  - Blood. 2009 Nov 12;114(20):4411-21. doi: 10.1182/blood-2008-11-188045. Epub 2009 
      Sep 18.