PMID- 1976759
OWN - NLM
STAT- MEDLINE
DCOM- 19901120
LR  - 20220309
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 55
IP  - 5
DP  - 1990 Nov
TI  - The effects of opioid peptides on dopamine release in the nucleus accumbens: an 
      in vivo microdialysis study.
PG  - 1734-40
AB  - An involvement of the mesolimbic dopamine (DA) system in mediating the 
      motivational effects of opioids has been suggested. Accordingly, the present 
      study employed the technique of in vivo microdialysis to examine the effects of 
      selective mu-, delta-, and kappa- opioids on DA release in the nucleus accumbens 
      (NAC) of anesthetized rats. Microdialysis probes were inserted into the NAC and 
      perfusates were analyzed for DA and its metabolites, dihydroxyphenylacetic acid 
      (DO-PAC) and homovanillic acid (HVA), using a reverse-phase HPLC system with 
      electrochemical detection for separation and quantification. 
      Intracerebroventricular (i.c.v.) administration of selective mu-opioid [D-Ala2, 
      N-methyl-Phe4, Gly5-ol]-enkephalin (DAMGO) or delta-opioid [D-Pen2, 
      D-Pen5]-enkephalin (DPDPE) agonists, at doses that function as positive 
      reinforcers in rats, resulted in an immediate and significant increase in 
      extracellular DA. DOPAC and HVA levels were also significantly increased. The 
      effects of DAMGO were blocked by the selective mu-antagonist 
      D-Pen-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2 (CTOP) whereas those of DPDPE were 
      blocked by the delta-antagonist allyl2-Tyr-Aib-Aib-Phe-Leu-OH (ICI 174,864). In 
      contrast to mu- and delta-agonists, the kappa-agonist 
      N-CH3-Tyr-Gly-Gly-Phe-Leu-Arg-N-CH3-Arg-D-Leu-NHC2H5 (E-2078), a dynorphin analog 
      that produces aversive states, decreased DA release in a biphasic manner. 
      Norbinaltorphimine, a selective kappa-antagonist, could block this effect. These 
      results demonstrate that mu-, delta-, and kappa-opioid agonists differentially 
      affect DA release in the NAC and this action is centrally mediated.
FAU - Spanagel, R
AU  - Spanagel R
AD  - Department of Neuropharmacology, Max-Planck-Institut fur Psychiatrie, 
      Planegg-Martinsried, F.R.G.
FAU - Herz, A
AU  - Herz A
FAU - Shippenberg, T S
AU  - Shippenberg TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Analgesics)
RN  - 0 (Endorphins)
RN  - 0 (Enkephalins)
RN  - 0 (Peptide Fragments)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 103613-84-9 (E 2078)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 74913-18-1 (Dynorphins)
RN  - 88373-73-3 (Enkephalin, D-Penicillamine (2,5)-)
RN  - I38ZP9992A (Magnesium)
RN  - RWP5GA015D (Potassium)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Analgesics/pharmacology
MH  - Animals
MH  - Dialysis/methods
MH  - Dopamine/*metabolism
MH  - Dynorphins/analogs & derivatives/pharmacology
MH  - Endorphins/*pharmacology
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
MH  - Enkephalin, D-Penicillamine (2,5)-
MH  - Enkephalins/pharmacology
MH  - Injections, Intraventricular
MH  - Magnesium/pharmacology
MH  - Male
MH  - Nucleus Accumbens/*metabolism
MH  - Peptide Fragments/pharmacology
MH  - Potassium/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Tetrodotoxin/pharmacology
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.1111/j.1471-4159.1990.tb04963.x [doi]
PST - ppublish
SO  - J Neurochem. 1990 Nov;55(5):1734-40. doi: 10.1111/j.1471-4159.1990.tb04963.x.